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| 当归补血汤提取物亚慢性毒性试验研究 | |
| 引用本文： | 李旭廷,王斌,李思聪,杨锐,李金良,张敏.当归补血汤提取物亚慢性毒性试验研究[J].中国畜牧兽医,2017,44(10):3090-3099. |
| 作者姓名： | 李旭廷 王斌 李思聪 杨锐 李金良 张敏 |
| 作者单位： | 1. 四川省畜牧科学研究院兽药研究所, 成都 610066; 2. 动物遗传育种四川省重点实验室, 成都 610066 |
| 基金项目： | 四川省科技支撑计划（2015NZ0018）；四川省基本科研业务费项目（SASA2013B07）；四川省财政运行专项（SASA2014CZYX010） |
| 摘 要： | 试验旨在评价当归补血汤提取物安全性，为临床安全用药提供试验依据。选取120只SD大鼠（雌雄各半）随机分为高、中、低剂量组和空白对照组，药物组分别按60、30、15 g/kg体重剂量灌胃给药，空白对照组给予等体积蒸馏水，连续给药28 d，停药后观察14 d。结果显示，给药结束时，高剂量组雄性大鼠出现体重增长减缓（P < 0.01）和饲料消耗量降低（P < 0.01），雄性大鼠白细胞数（WBC）、淋巴细胞（Lym）均显著升高（P < 0.05），丙氨酸氨基转移酶（ALT）、总胆固醇（TC）、葡萄糖（GLU）均极显著降低（P < 0.01），血小板（PLT）显著减少（P < 0.05）；雌性大鼠红细胞（RBC）、血红蛋白（HGB）、ALT均极显著减少（P < 0.01）。中剂量组大鼠ALT和碱性磷酸酶（ALP）显著或极显著降低（P < 0.05；P < 0.01）。停药第14天雄性大鼠网织红细胞（RET）显著增多（P < 0.05），雌性大鼠HGB显著减少（P < 0.05）。停药后可恢复正常，其余相关检测指标均未见异常。各脏器组织病理学检查均未见异常。结果表明当归补血汤提取物毒性低，可以安全用于动物。 |
| 关 键 词： | 当归补血汤 大鼠 亚慢性毒性 |
| 收稿时间： | 2017-03-16 |
Study on Subacute Toxicity Test of the Extact of Dangguibuxue Tang |
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| LI Xu-ting,WANG Bin,LI Si-cong,YANG Rui,LI Jin-liang,ZHANG Min.Study on Subacute Toxicity Test of the Extact of Dangguibuxue Tang[J].China Animal Husbandry & Veterinary Medicine,2017,44(10):3090-3099. | |
| Authors: | LI Xu-ting WANG Bin LI Si-cong YANG Rui LI Jin-liang ZHANG Min |
| Institution: | 1. Institute of Veterinary Pharmacology, Sichuan Animal Science Academy, Chengdu 610066, China; 2. Sichuan Key Laboratory of Animal Genetics and Breeding, Chengdu 610066, China |
| Abstract: | To evaluate the safety of the Dangguibuxue Tang (DBT) for animals, SD rats were administrated DBT by gavage once daily at 0, 15, 30 and 60 g/(kg·BW) for 28 consecutive days as control, low, medium and high dosage groups, and followed by a 14-day recovery period. The results showed that compared with control group, the weight gain and food intake of male rats in the high dosage group were extremely significantly decreased (P < 0.01). WBC and Lym of male rats in the high dosage group were significantly increased (P < 0.05), whereas ALT, TC and GLU were extremely significantly decreased (P < 0.01),PLT was significantly decreased (P<0.05). RBC, HGB and ALT of female rats in high dosage group were extremely significantly decreased (P < 0.01). ALT and ALP of rats in medium dosage group were significantly or extremely significantly decreased (P < 0.05;P < 0.01). While in medium dosage group RET of male rats was significantly increased (P < 0.05) and HGB of the female rats were significantly decreased (P < 0.05) 14 days after withdrawal. However, these indexes had no significant difference to control group at the end of recovery period. In addition, no changes were observed in the main organs of rats at macroscopic and microscopic level. The result showed that subacute administration of DBT was not toxic in SD rats, suggesting a safety use for animals. |
| Keywords: | Dangguibuxue Tang rat subacute toxicity |
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