| 新型多肽表位技术研究进展 |
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| 引用本文： | 韩勇,高花,翟晓鑫,高凤山.新型多肽表位技术研究进展[J].中国畜牧兽医,2017,44(1):44-52. |
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| 作者姓名： | 韩勇 高花 翟晓鑫 高凤山 |
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| 作者单位： | 大连大学生命科学与技术学院, 分子免疫学实验室, 大连 116622 |
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| 基金项目： | 国家自然科学基金项目：猪源病毒CTL多肽表位与SLA-Ⅰ结晶研究（31172304） |
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| 摘 要： | 多肽表位作为抗原的重要组成成分，可以被宿主免疫系统B细胞产生的抗体（Ig）和T细胞受体（TCR）识别，从而诱导机体产生免疫保护作用。传统的研究方法如交叠肽合成等费时费力，极大地限制了多肽表位的研究。随着生物信息学、高通量测序、CRISPR/Cas9、质谱（MS）等技术出现以及TAP非依赖型蛋白加工机制的不断阐述，使表位的研究得到不断完善。作者介绍了最新的多肽表位研究方法，包括利用计算机对B细胞和T细胞表位的预测、基于MHC与抗原肽结构作用为基础的方法、结合高通量测序技术的表位筛选方法、结合新型基因编辑技术的表位筛选方法、TAP非依赖性T细胞表位的最新研究等，并对今后多肽表位研究的发展进行了展望。
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| 关 键 词： | 多肽 表位 筛选 CRISPR/Cas9技术 TAP非依赖性表位 |
| 收稿时间： | 2016-06-30 |
Research Progress on Novel Peptide Epitopes Technology |
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| HAN Yong,GAO Hua,ZHAI Xiao-xin,GAO Feng-shan.Research Progress on Novel Peptide Epitopes Technology[J].China Animal Husbandry & Veterinary Medicine,2017,44(1):44-52. |
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| Authors: | HAN Yong GAO Hua ZHAI Xiao-xin GAO Feng-shan |
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| Institution: | Laboratory of Moleculer Immunology, College of Life Science and Technology, Dalian University, Dalian 116622, China |
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| Abstract: | As an important component of the antigen, peptide epitopes can be recognized by T cell receptor (TCR) and antibodies which induced protective immunity by the host immune system. Traditional research methods, such as overlapping peptide synthesis is long time-consuming which greatly limits the study of epitopes. With the development of bioinformatics, high-throughput sequencing, CRISPR/Cas9 technology, MS and other technologies and explaining the processing mechanism of TAP-dificient protein constantly, the research for epitopes is being perfect continually. In this article, some new methods to study epitopes were introduced, including computer prediction of B cell or T cell epitopes, identification of epitopes based on the structure and interaction of MHC and antigenic peptides, screening epitopes by means of high throughput sequencing, screening epitopes by means of newly genome editing, the up-to-date research in TAP-deficient T cell epitopes, etc. Finally,future prospect for research direction of peptides was further forecasted. |
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| Keywords: | peptide epitope screening CRISPR/Cas9 technology TAP-dificient epitopes |
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