| 添加通路阻断剂对IGF-1影响奶牛乳腺上皮细胞增殖与凋亡的研究 |
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| 引用本文： | 雒诚龙,赵艳坤,武开乐,邵伟.添加通路阻断剂对IGF-1影响奶牛乳腺上皮细胞增殖与凋亡的研究[J].中国畜牧兽医,2017,44(8):2277-2282. |
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| 作者姓名： | 雒诚龙 赵艳坤 武开乐 邵伟 |
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| 作者单位： | 新疆农业大学动物科学学院, 乌鲁木齐 830052 |
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| 基金项目： | 国家自然科学基金（31560645）；新疆维吾尔自治区重点实验室开放课题（2015KL018）；中国博士后基金委资助 |
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| 摘 要： | 试验旨在研究胰岛素样生长因子-1（insulin-like growth factor 1，IGF-1）对奶牛乳腺上皮细胞（bovine mammary epithelial cells，BMECs）增殖的影响，为后期利用IGF-1调控乳腺发育奠定理论基础。以奶牛乳腺上皮细胞为材料，首先分4组分别外源添加0（对照组）、10、50、100 μg/mL IGF-1且分别培养12、24、48、72 h，测定抑制BMECs凋亡率的最佳浓度；然后分6组：单纯BMECs组、BMECs+IGF-1组、BMECs+LY294002组、BMECs+IGF-1+LY294002组、BMECs+RAPA组和BMECs+IGF-1+RAPA组，采用流式细胞术测定各组细胞凋亡率。结果表明，外源性添加IGF-1对BMECs凋亡率具有抑制作用，最佳浓度为50 μg/mL；BMECs+IGF-1+LY294002与BMECs+IGF-1+RAPA组细胞凋亡率均极显著高于BMECs+IGF-1组（P<0.01）。推断IGF-1能够活化PI3K/Akt/mTOR信号通路，参与BMECs凋亡的调控作用，进而抑制BMECs细胞凋亡；IGF-1可能会对被抑制PI3K/Akt/mTOR信号通路产生"修复"机制，使其能够重新参与BMECs的生命进程。
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| 关 键 词： | 胰岛素样生长因子-1(IGF-1) 奶牛乳腺上皮细胞(BMECs) 增殖 凋亡 |
| 收稿时间： | 2017-04-06 |
Study on Effect of IGF-1 on Proliferation and Apoptosis of Bovine Mammary Epithelial Cells by Adding Inhibitor |
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| LUO Cheng-long,ZHAO Yan-kun,WU Kai-le,SHAO Wei.Study on Effect of IGF-1 on Proliferation and Apoptosis of Bovine Mammary Epithelial Cells by Adding Inhibitor[J].China Animal Husbandry & Veterinary Medicine,2017,44(8):2277-2282. |
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| Authors: | LUO Cheng-long ZHAO Yan-kun WU Kai-le SHAO Wei |
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| Institution: | College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | The test was aimed to study the effect of insulin-like growth factor 1(IGF-1) on the proliferation of bovine mammary epithelial cells (BMECs),and provide a theoretical basis for the subsequent regulating mammary gland development using IGF-1. The optimal IGF-1 concentration for inhibiting BMECs apoptosis was obtained by measuring the apoptosis rate at 12, 24, 48 and 72 h after the addition of IGF-1 at four groups (0, 10, 50 and 100 μg/mL). Then divided into six groups:BMECs group, BMECs+IGF-1 group, BMECs+LY294002 group, BMECs+IGF-1+LY294002 group, BMECs+RAPA group and BMECs+IGF-1+RAPA group,and the apoptosis rates were detected by flow cytometry. The results showed that the heterologous IGF-1 had an inhibitory effect on the apoptosis of BMECs with an optimal concentration of 50 μg/mL. The apoptosis rates of BMECs+IGF-1+LY294002 and BMECs+IGF-1+RAPA groups were extremely significantly higher than BMECs+IGF-1 group (P<0.01). This study suggested that IGF-1 could activate the PI3K/Akt/mTOR signaling pathway and thereby inhibit the apoptosis of BMECs. Moreover, IGF-1 might also promote the "repair" mechanism for the inhibited PI3K/Akt/mTOR signaling pathway, and therefore make it reparticipate in BMECs life process. |
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| Keywords: | insulin-like growth factor 1(IGF-1) bovine mammary epithelial cells (BMECs) proliferation apoptosis |
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