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| 不同品种猪感染HP-PRRSV后肺组织间PRRSV受体基因差异表达的研究 | |
| 引用本文： | 康润敏,姬高升,曾凯,吕学斌,殷明郁.不同品种猪感染HP-PRRSV后肺组织间PRRSV受体基因差异表达的研究[J].中国畜牧兽医,2017,44(2):538-545. |
| 作者姓名： | 康润敏 姬高升 曾凯 吕学斌 殷明郁 |
| 作者单位： | 1. 四川省畜牧科学研究院, 动物遗传育种四川省重点实验室, 成都 610066; 2. 四川大学生命科学学院, 动物疫病防控与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室, 成都 610064 |
| 基金项目： | 四川公益性科研院所基本科研项目（SASA2015A03）；四川省科技支撑计划（2014NZ009、16ZC2850）；国家生猪产业技术体系（CARS-36） |
| 摘 要： | 为进一步了解藏猪、藏梅猪和大约克夏猪对高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV） JXA1分离株具有不同易感性的分子机理，本研究建立实时荧光定量RT-PCR方法比较分析藏猪、藏梅猪和大约克夏猪感染JXA1分离株前、感染后4、7和14 d肺脏中HSPG2、SIGLEC1、CD163、VIM和NMMHC-ⅡA 5个PRRSV受体基因的差异表达情况。结果显示，藏猪感染JXA1分离株后4和14 d肺脏中HSPG2的表达量显著高于感染前（P < 0.05），而感染后7 d HSPG2的表达量显著低于感染前（P < 0.05），藏梅猪在感染后14 d HSPG2的表达量显著高于感染后7 d（P < 0.05）；藏猪感染后4和14 d肺脏中SIGLEC1的表达量显著高于感染前（P < 0.05），大约克夏猪感染后4、7和14 d SIGLEC1的表达量均显著低于感染前（P < 0.05）；藏猪和藏梅猪感染后14 d肺脏中CD163的表达量均显著高于感染前（P < 0.05），大约克夏猪则感染后7和14 d均显著低于感染前（P < 0.05）；藏猪感染后7 d肺脏中VIM的表达量显著高于感染前（P < 0.05），大约克夏猪在感染后7 d显著低于感染前（P < 0.05）；藏梅猪感染后4 d肺脏中NMMHC-ⅡA的表达量显著高于感染前（P < 0.05），大约克夏猪在感染后4和14 d NMMHC-ⅡA的表达量显著高于感染前（P < 0.05）。结果表明，5个PRRSV受体基因中，SIGLEC1和VIM基因可能是影响藏猪、藏梅猪和大约克夏猪对JXA1分离株易感性存在差异的潜在关键基因。 |
| 关 键 词： | JXA1分离株 藏猪 藏梅猪 大约克夏猪 PRRSV受体基因 差异表达 |
| 收稿时间： | 2016-08-12 |
Study on Differential Expression of PRRSV Receptor Genes in Lung Tissues of Different Breeds of Pig Infected with HP-PRRSV |
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| KANG Run-min,JI Gao-sheng,ZENG Kai,LV Xue-bin,YIN Ming-yu.Study on Differential Expression of PRRSV Receptor Genes in Lung Tissues of Different Breeds of Pig Infected with HP-PRRSV[J].China Animal Husbandry & Veterinary Medicine,2017,44(2):538-545. | |
| Authors: | KANG Run-min JI Gao-sheng ZENG Kai LV Xue-bin YIN Ming-yu |
| Institution: | 1. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, China; 2. Key Laboratory of Bio-resources and Ecoenvironment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, China |
| Abstract: | In order to understand the molecular mechanism of host susceptibility differential to highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRSV) infection among three breeds of pig, Tibetan pig,ZangMei Black and Yorkshire,the mRNA level of five reported PRRSV receptor genes (HSPG2,SIGLEC1,CD163,VIM and NMMHC-ⅡA) expressed in lung were investigated on 0,4, 7 and 14 days post infection (dpi) with HP-PRRSV JXA1 isolate.The Real-time RT-PCR results showed that the expression of HSPG2 in Tibetan pig increased significantly on 4 and 14 dpi and decreased significantly on 7 dpi compared with the expression on 0 dpi (P < 0.05), and its expression in ZangMei Black on 14 dpi was significantly higher than that of on 7 dpi (P < 0.05);An increased expression of SIGLEC1 in Tibetan pig was observed on 4 and 14 dpi (P < 0.05), and a decreased expression of the same gene was detected in Yorkshire on 4,7 and 14 dpi (P < 0.05);The expression of CD163 elevated significantly on 14 dpi in both Tibetan pig and ZangMei Black (P < 0.05), however its expression reduced significantly on 7 and 14 dpi in Yorkshire (P < 0.05); The expression of VIM gene was significantly higher on 7 dpi in Tibetan pig and significantly lower on 7 dpi in Yorkshire (P < 0.05); And the expression of NMMHC-ⅡA was significantly higher on 4 dpi in ZangMei Black, which was also significantly higher in Yorkshire on 4 and 14 dpi (P < 0.05).In conclusion, SIGLEC1 and VIM might be important genes to influence the host resistance of Tibetan pig,ZangMei Black and Yorkshire to HP-PRRSV infection. |
| Keywords: | JXA1 isolate Tibetan pig ZangMei Black Yorkshire PRRSV receptor genes differential expression |
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