首页 | 本学科首页   官方微博 | 高级检索  
     检索      

水貂志贺氏菌分离鉴定及药物敏感性分析
引用本文:孙娜,陈强,杨艳玲,郭利,冯秋菊,孙铭,程世鹏.水貂志贺氏菌分离鉴定及药物敏感性分析[J].中国畜牧兽医,2017,44(11):3379-3384.
作者姓名:孙娜  陈强  杨艳玲  郭利  冯秋菊  孙铭  程世鹏
作者单位:1. 中国农业科学院特产研究所, 农业部经济动物疫病重点实验室, 长春 130112;
2. 吉林特研生物技术有限责任公司, 长春 130112;
3. 四川省凉山州木里县农牧局, 木里 615899;
4. 吉林省通化市动物疫病预防控制中心, 通化 134000
基金项目:吉林省科技发展计划项目(20150520128JH);中国农业科学院创新工程基金(20140204066NY);公益性行业(农业)科研专项经费项目任务书(201303042)
摘    要:试验旨在对吉林省某水貂养殖场送检的3只具有典型腹泻症状的病死水貂的小肠和肠内容物样品进行细菌分离鉴定及耐药情况分析,为临床治疗提供参考。通过细菌分离纯化和PCR方法对分离菌株进行鉴定,对BALB/c小鼠进行菌液注射来检测菌株的致病性。采用K-B药敏法检测菌株对常用药物的敏感性,并通过PCR方法检测其耐药基因和Ⅰ类整合子的携带情况。结果显示,分离得到3株志贺氏菌,致病性检测试验显示分离菌可引起小鼠腹泻。药物敏感性试验结果显示,3株志贺氏菌对氟喹诺酮类药物、头孢类药物、氟苯尼考和多黏菌素较敏感,对氨基糖苷类、四环素、氯霉素、青霉素、氨苄西林耐药。耐药基因检测结果显示,3株志贺氏菌共检测出7种耐药基因blaTEM-1、aadA1、aac(3')-Ⅱc、aac(6')-Ⅰb、aph(3')-Ⅶ、tet(M)、cat2及携带aadA1基因盒的Ⅰ类整合子。结果表明,分离的3株志贺氏菌均具有致病性,可引起小鼠腹泻,主要表现为多重耐药现象,携带的耐药基因呈多样性,为临床治疗带来巨大影响。

关 键 词:水貂  志贺氏菌  腹泻  药物敏感性  
收稿时间:2017-05-12

Isolation,Identification and Drug Sensitivity Analysis of Shigella from Minks
SUN Na,CHEN Qiang,YANG Yan-ling,GUO Li,FENG Qiu-ju,SUN Ming,CHENG Shi-peng.Isolation,Identification and Drug Sensitivity Analysis of Shigella from Minks[J].China Animal Husbandry & Veterinary Medicine,2017,44(11):3379-3384.
Authors:SUN Na  CHEN Qiang  YANG Yan-ling  GUO Li  FENG Qiu-ju  SUN Ming  CHENG Shi-peng
Abstract:In order to provide therapeutical guidance for drug admistration, the bacteria of three sick minks suffering from typical diarrhea symptoms provided by mink farms in Jilin province were isolated and identified, and the drug sensitivity was tested. The bacteria were isolated with TSA plates, and identified using biochemical methods and PCR assay. The virulence of the isolates was determined by infecting BALB/c mice. The antimicrobial susceptibility of the isolates to antimicrobial agents was investigated using the K-B method. PCR was used to detect the resistance genes and Ⅰ integrons. A total of 3 Shigella isolates were obtained from sick minks. The virulent determination showed that all isolates could cause mice diarrhea. The drug sensitivity results showed that 3 strains were sensitive to fluoroquinolone, cephalosporin, florfenicol and polymyxin, but they were resistant to aminoglycoside, tetracycline, chloramphenicol, penicillin and ampicillin. There were seven resistance genes were detected,blaTEM-1,aadA1, aac(3')-Ⅱc, aac(6')-Ⅰb, aph(3')-Ⅶ, tet(M), cat2 and three class Ⅰ integrons carrying aadA 1 gene cassette. All of the isolates were virulent and caused the mice diarrhea. The resistance of the 3 strains were very serious and mainly for multiple drug resistance phenomenon. The resistance genes detected in the mink were various, and could bring enormous implications for clinical treatment.
Keywords:mink  Shigella  diarrhea  drug sensitivity  
本文献已被 CNKI 等数据库收录!
点击此处可从《中国畜牧兽医》浏览原始摘要信息
点击此处可从《中国畜牧兽医》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号