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| 北京地区仔猪大肠杆菌血清型鉴定及其毒力因子检测 | |
| 引用本文： | 王振玲,段跃强,王金秋,王宇.北京地区仔猪大肠杆菌血清型鉴定及其毒力因子检测[J].中国畜牧兽医,2017,44(5):1302-1308. |
| 作者姓名： | 王振玲 段跃强 王金秋 王宇 |
| 作者单位： | 1. 北京农业职业学院, 北京 102442; 2. 中国人民解放军军事医学科学院, 北京 100071; 3. 内蒙古农业大学, 呼和浩特 010018 |
| 基金项目： | 北京市“菜篮子”科技能力提升工程项目；北京农业职业学院院级项目 |
| 摘 要： | 本试验旨在调查北京地区仔猪腹泻的原因及猪源大肠杆菌血清型、毒力因子的分布情况。收集北京京郊地区仔猪腹泻样本400份，应用含2%血清的TSA培养基分离大肠杆菌，通过血清型鉴定试验和毒力因子检测验证O抗原。结果显示，400份样本中分离到64株大肠杆菌，定型42株。其主要为O101型（18.7%）、O64型（12.5%）、O8型（10.9%）、O20型（10.9%）、O45型（4.7%）、O149型（4.7%）、O2型（1.6%）、O89型（1.6%）8种血清型，其携带的毒力因子为STa、Stx2e、astA和eaeA等。结果表明，引起京郊仔猪腹泻的大肠杆菌主要有8个血清型，其中O101型发病比例最高。其携带的毒力因子主要为astA和eaeA，占全部菌株的50%以上，STa和Stx2e因子占全部菌株的30%以上。本试验结果将为今后京郊地区仔猪腹泻病的防控提供有效的数据支持。 |
| 关 键 词： | 仔猪腹泻病 大肠杆菌 血清型 毒力因子 |
| 收稿时间： | 2016-09-05 |
Detection of Serotypes and Virulence Factors of Escherichia coli from Piglets in Beijing |
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| WANG Zhen-ling,DUAN Yue-qiang,WANG Jin-qiu,WANG Yu.Detection of Serotypes and Virulence Factors of Escherichia coli from Piglets in Beijing[J].China Animal Husbandry & Veterinary Medicine,2017,44(5):1302-1308. | |
| Authors: | WANG Zhen-ling DUAN Yue-qiang WANG Jin-qiu WANG Yu |
| Institution: | 1. Beijing Vocational College of Agriculture, Beijing 102442, China; 2. Chinese PLA Military Medical Science Academy of the PLA, Beijing 100071, China; 3. Inner Mongolia Agricultural University, Hohhot 010018, China |
| Abstract: | To investigate the cause of piglets diarrhea, and the distribution of the serotype and virulence factors of swine Escherichia coli in Beijing, 400 diarrhea samples were collected. TSA serum agar culture method was used to isolate Escherichia coli, serotype identification test and virulence factor genes test were used to verify the presence of O-antigen. 64 strains of E.coli were isolated from 400 diarrhea samples, among which 42 strains of E.coli were classified as 8 serotypes:O101(18.7%),O64(12.5%),O8(10.9%),O20(10.9%),O45(4.7%),O149(4.7%),O2(1.6%) and O89(1.6%), and the major virulence factors were STa, Stx2e, astA and eaeA. There were 8 mainly serotypes that caused piglets diarrhea in Beijing area, among which O101 serotype accounted for the highest proportion. The major virulence factors were astA and eaeA, accounted for more than 50% of all strains, STa and Stx2e accounted for more than 30% of all strains. These data would provide effective data to support the prevention and control of piglet diarrhea in Beijing. |
| Keywords: | piglet diarrhea Escherichia coli serotype virulence factor |
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