| 金荞麦超微粉对肉鸡免疫功能、生长发育的影响 |
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| 引用本文： | 胡逸枫,梁夏瑜,王航,汤承,岳华.金荞麦超微粉对肉鸡免疫功能、生长发育的影响[J].中国畜牧兽医,2017,44(11):3195-3200. |
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| 作者姓名： | 胡逸枫 梁夏瑜 王航 汤承 岳华 |
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| 作者单位： | 西南民族大学药学院, 成都 610041 |
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| 基金项目： | 中央高校基本科研项目（2017NZYQN13） |
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| 摘 要： | 本试验为研究金荞麦超微粉对肉鸡免疫功能和生长发育的影响，将160只1日龄白羽肉鸡随机分为4组，7日龄时用新城疫活疫苗滴鼻（1头份/只），同时颈部皮下注射新城疫-禽流感二联灭活疫苗（0.3 mL/只），间隔12 h后各组分别饲喂含不同药物的饲料，自由采食。低、高剂量组分别在饲料中添加4.50‰、8.75‰金荞麦超微粉，连续给药7 d。阳性对照组在饲料中添加400 g/L黄芪多糖，连续给药7 d；空白对照组饲料中不添加任何药物。随后在给药结束后的第7、21和35天翅下静脉采血用于分离淋巴细胞和血清，用CCK-8法测定外周血淋巴细胞增殖能力；微量血凝抑制试验评价血清中抗体效价；同时称量各组肉鸡体重，屠宰后解剖，取脾脏、法氏囊和胸腺计算免疫器官指数。结果显示，与对照组相比，在给药结束后7、21和35 d，金荞麦超微粉高、低剂量组与黄芪多糖组料重比均不同程度降低（P>0.05）；此外给药组可提高肉鸡外周血淋巴细胞增殖能力、免疫抗体水平和肉鸡体重，其中给药结束后21 d，金荞麦超微粉高剂量组禽流感病毒和新城疫病毒抗体效价与对照组相比差异极显著（P<0.01）；同时还能提高脾脏和法氏囊的脏器系数，并具有量效关系。综上所述，金荞麦超微粉可以显著提高雏鸡的细胞免疫、体液免疫和非特异性免疫功能，并显著增加体重。表明金荞麦超微粉具有增强白羽肉鸡免疫力和促进生长发育的功效，为开发金荞麦超微粉为新型天然植物药、饲料添加剂提供了依据。
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| 关 键 词： | 金荞麦超微粉 肉鸡 免疫功能 生长发育 |
| 收稿时间： | 2017-05-22 |
Effect of Fagopyrum dibotryis Superfine Powder on the Immune Function and the Growth and Development of Broilers |
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| HU Yi-feng,LIANG Xia-yu,WANG Hang,TANG Cheng,YUE Hua.Effect of Fagopyrum dibotryis Superfine Powder on the Immune Function and the Growth and Development of Broilers[J].China Animal Husbandry & Veterinary Medicine,2017,44(11):3195-3200. |
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| Authors: | HU Yi-feng LIANG Xia-yu WANG Hang TANG Cheng YUE Hua |
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| Institution: | Southwest University for Nationalities, Chengdu 610041, China |
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| Abstract: | In order to study the effects of Fagopyrum dibotryis superfine powder on the immune function and growth and development of broilers,160 one-day-old White feather broilers were chosen and randomly divided into 4 groups. The nasal drops were given with Newcastle disease live vaccine at 7 days old,and Newcastle disease-avian influenza inactivated vaccine was given by subcutaneous injection (0.3 mL/only).After 12 h,each group was fed with different feeds,the broilers in the low and high dose groups were fed diet supplemented 4.50‰,8.75‰ Fagopyrum dibotryis superfine powder,respectively,administration for 7 d. The broilers in the positive control group were fed diet added 400 g/L Astragalus polysaccharide, continuous administration for 7 d,and feed of blank control group did not add any drugs. The lymphocytes and serum were collected and then used for the separation of lymphocytes and serum at 7,21 and 35 d after the end of the administration. The proliferation of peripheral blood lymphocytes was measured by CCK-8 method. The titer of serum was evaluated by the hemagglutination inhibition test.The body weight of each group was weighed at the same time.After that,the rats were sacrificed,the spleen and bursa of Fabricius were used to calculate the immune organ index. The results showed that compared with the control group,the F/G of high dose group,low dose group and Astragalus polysaccharide group were decreased by different degrees at 7,21 and 35 d after the end of the administration (P>0.05). In addition,the proliferation ability of the peripheral blood lymphocytes,immune antibody level and the body weight in broilers of administration groups were increased,in which the titers of AIV and NDV antibodies were extremely significantly increased in high dose group compared with the control group (P<0.01).Meanwhile the Fagopyrum dibotryis superfine powder also could increase the spleen and bursa of Fabricius indexes. In conclusion,the Fagopyrum dibotryis superfine powder could significantly improve the cellular immunity, humoral immunity and nonspecific immune function of broilers,and significantly increased the body weight. According to the specification of the results of the instructions, we could determine that it had the effect to comprehensively improve the immunity and growth of White feather broilers. The study could lay the foundation of the future development of Fagopyrum dibotryis superfine powder into a new natural herbal medicine and feed additives. |
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| Keywords: | Fagopyrum dibotryis superfine powder broilers immune function growth and development |
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