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| 产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制 | |
| 引用本文： | 李海花,朱琪,王世琼,杨春蕾,赵向华,乔家运,王文杰.产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制[J].中国畜牧兽医,2017,44(1):262-267. |
| 作者姓名： | 李海花 朱琪 王世琼 杨春蕾 赵向华 乔家运 王文杰 |
| 作者单位： | 1. 天津市畜牧兽医研究所, 天津 300381; 2. 天津市畜禽健康养殖技术工程中心, 天津 300381 |
| 基金项目： | 国家自然科学基金（31402087） |
| 摘 要： | 为探讨产肠毒素大肠杆菌（ETEC） K88感染仔猪发生炎症反应的分子机制，试验用ETEC K88灌服断奶仔猪，ELISA法检测攻毒后仔猪血清中白细胞介素8（IL-8）含量，实时荧光定量PCR方法检测淋巴结中Toll样受体2（TLR2）、Toll样受体4（TLR4）及其信号通路相关基因（髓样分化因子88（MyD88）、Toll相互作用蛋白（Tollip）、B细胞淋巴瘤因子3（Bcl3））的mRNA相对表达水平。结果发现，仔猪攻毒ETEC K88后6和24 h血清IL-8含量和淋巴结TLR2/4的表达水平均极显著或显著高于对照组（P<0.01；P<0.05），且感染后24 h显著低于感染后6 h（P<0.05）；仔猪感染ETEC K88后24 h淋巴结中MyD88、Tollip和Bcl3的表达水平均极显著高于对照组（P<0.01），但是感染后6 h时与对照组相比均无显著差异（P>0.05）。综上所述，ETEC K88感染仔猪可能是通过TLR2/4-MyD88信号通路产生炎症因子IL-8，促使仔猪出现炎症反应，且该炎症反应可能受Tollip和Bcl3蛋白的调控而被减弱。 |
| 关 键 词： | 大肠杆菌K88 仔猪 炎症反应 Toll-样受体 炎症调控 |
| 收稿时间： | 2016-06-14 |
Molecular Mechanism of the Inflammatory Response Induced by Enterotrxigenic E. coli K88 in Piglets |
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| LI Hai-hua,ZHU Qi,WANG Shi-qiong,YANG Chun-lei,ZHAO Xiang-hua,QIAO Jia-yun,WANG Wen-jie.Molecular Mechanism of the Inflammatory Response Induced by Enterotrxigenic E. coli K88 in Piglets[J].China Animal Husbandry & Veterinary Medicine,2017,44(1):262-267. | |
| Authors: | LI Hai-hua ZHU Qi WANG Shi-qiong YANG Chun-lei ZHAO Xiang-hua QIAO Jia-yun WANG Wen-jie |
| Institution: | 1. Tianjin Institute of Animal Husbandry and Veterinary Science, Tianjin 300381, China; 2. Tianjin Engineering Research Center for Livestock and Poultry Health Breeding, Tianjin 300381, China |
| Abstract: | To investigate the molecular mechanism of the inflammatory response in the piglets infected with enterotoxigenic E. coli (ETEC) K88, piglets were infected with ETEC K88,the IL-8 content in serum of piglets were assayed by ELISA,and the mRNA relative expression levels of TLR2/4 and its signal transduction pathway related genes (MyD88,Tollip and Bcl3) in mesenteric lymph nodes were detected by quantitative Real-time PCR. The results showed that compared with control group,the content of IL-8 in serum and the expressions of TLR2/4 in lymph nodes were all extremely significantly or significantly increased at 6 and 24 h after infection (P<0.01;P<0.05),and the IL-8 content and TLR2/4 mRNA expression at 24 h after infection were all significantly lower than those at 6 h after infection (P<0.05).In addition,the expressions of MyD88,Tollip and Bcl3 in lymph nodes were all extremely significantly increased at 24 h after infection compared with control group (P<0.01), but there was no significant difference between experimental group and control group at 6 h after infection (P>0.05). In conclusion,ETEC K88 infected piglets might produce inflammatory cytokines IL-8 through the TLR2/4-MyD88 signaling pathway,which could promote the inflammatory reaction in piglets. This inflammatory response might be regulated by Tollip and Bcl3,which could weak the inflammatory intensity in piglets. |
| Keywords: | E coli K88 piglet inflammatory response Toll-like receptors inflammatory regulation |
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