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| 获得最大酵母多糖提取率的破壁方法研究 | |
| 引用本文： | 李晶晶,冀国珍,高爱武,曹平,杨金丽,王海荣.获得最大酵母多糖提取率的破壁方法研究[J].中国畜牧兽医,2017,44(3):651-658. |
| 作者姓名： | 李晶晶 冀国珍 高爱武 曹平 杨金丽 王海荣 |
| 作者单位： | 1. 内蒙古农业大学动物科学学院, 动物营养实验室, 呼和浩特 010018; 2. 内蒙古农业大学食品科学学院, 呼和浩特 010018; 3. 内蒙古农业大学乳品重点实验室, 呼和浩特 010018 |
| 基金项目： | 自治区科技创新引导奖励资金项目（20140608）；反刍动物新型复合微生态制剂的创制与应用 |
| 摘 要： | 试验旨在选择获得最大酵母多糖提取率的酵母菌破壁方法。以酵母多糖提取率与细胞破壁率为综合评定指标，首先对超声波破碎、超声波清洗、反复冻融、蛋白酶处理4种方法进行条件优化，选择最适破壁条件；然后将此4种单一的破壁方法与超声波破碎和蛋白酶处理协同方法相比较，确定获得最大多糖提取率的破壁方法。结果表明，当超声波清洗时间达到40 min、超声波破碎时间达到50 min时，酵母多糖提取率达到最大值；当菌泥加水量和冻融次数分别为15%与3次时，多糖提取率最大；当蛋白酶用量300 IU/g、酶解时间15 h、pH 5.0、处理温度40℃时，蛋白酶处理的多糖提取率最高。4种破壁方法中蛋白酶处理的效果最佳，对应的多糖提取率为149.81 mg/g，破壁率为53.49%。协同破壁处理的提取效果要优于单一的破壁方法，但考虑到协同作用对多糖结构的完整性构成威胁，将蛋白酶处理确定为最佳的酵母菌破壁方法。 |
| 关 键 词： | 酵母菌 破壁方法 多糖提取率 破壁率 |
| 收稿时间： | 2016-06-17 |
The Study of Cell Wall Breaking Methods to Obtain Maximum Extraction Rate of Yeast Polysaccharides |
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| LI Jing-jing,JI Guo-zhen,GAO Ai-wu,CAO Ping,YANG Jin-li,WANG Hai-rong.The Study of Cell Wall Breaking Methods to Obtain Maximum Extraction Rate of Yeast Polysaccharides[J].China Animal Husbandry & Veterinary Medicine,2017,44(3):651-658. | |
| Authors: | LI Jing-jing JI Guo-zhen GAO Ai-wu CAO Ping YANG Jin-li WANG Hai-rong |
| Institution: | 1. Laboratory of Animal Nutrition, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China; 2. College of Food Science, Inner Mongolia Agricultural University, Hohhot 010018, China; 3. Key Laboratory of Dairy, Inner Mongolia Agricultural University, Hohhot 010018, China |
| Abstract: | The aim of this study was to determine the optimal method of the yeast cell wall breaking which could obtain the maximum extraction rate of yeast polysaccharide. The extraction rate of yeast polysaccharide and the cell wall broken rate were used as the evaluating indexes. The conditions of ultrasonic disruption, repeated freezing and thawing, ultrasonic cleaning and protease treatment were optimized to select the appropriate parameters in cell wall breaking. The effects of these 4 methods and the synergistic action of ultrasonic disruption and protease treatment were compared. The results showed that when the ultrasonic cleaning time was 40 min and ultrasonic disruption time was 50 min, the water content of slurry was 15% and the times of freezing and thawing was 3, and the protease treatment was carried out at 40℃ for 15 h with enzyme concentration 300 IU/g and pH 5.0, respectively, the extraction rates of yeast polysaccharides were the highest. The effect of protease treatment was the best among these 4 cell wall breaking methods, its extraction rate of polysaccharide was 149.81 mg/g and the cell wall broken rate was 53.49%. The effect of combined way was better than that of single treatment, but the synergistic action would destroy the integrity of polysaccharide's structure. So the protease treatment was thought an ideal cell wall breaking method of yeast. |
| Keywords: | yeast cell wall breaking methods polysaccharide extraction rate cell wall broken rate |
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