| 高致病性猪繁殖与呼吸综合征病毒样品对细胞的敏感性 |
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| 引用本文： | 张春玲,邹勇,张婉华,周宗清,李春华,蒋凤英.高致病性猪繁殖与呼吸综合征病毒样品对细胞的敏感性[J].浙江农业学报,2011,23(1):62. |
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| 作者姓名： | 张春玲 邹勇 张婉华 周宗清 李春华 蒋凤英 |
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| 作者单位： | 上海市农业科学院 畜牧兽医研究所,上海 201106 |
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| 基金项目： | 上海市农委重点攻关计划(沪农科攻字(2006)第10-4号) |
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| 摘 要： | 从疑似猪繁殖与呼吸综合征病毒(PRRSV)的发病猪场采集组织和血清样品并按病料分成2组,通过反转录—聚合酶链反应(RT-PCR)方法检测样品中高致病性PRRSV发生变异的Nsp2部分基因,初步筛选出PRRSV阳性样品后分别通过Marc-145和PAM细胞分离培养。结果表明：从45份阳性病料中成功分离到29份高致病性PRRSV,其中从血清样品中成功分离的约占总数72.5%,从组织样品中成功分离的约占总数27.5%；通过Marc-145细胞分离获得的病毒有19份,通过PAM细胞分离获得的有24份,其中有14份是Marc-145和PAM共同分离得到。试验获得的血清是高致病PRRSV分离的理想样品,该病毒对Marc-145和PAM细胞的敏感性差异不显著。
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| 关 键 词： | 高致病性PRRSV 细胞 敏感性分析 |
Sensitivity effect analysis of different highly pathogenic Porcine reproductive and respiratory syndrome virus samples on cells |
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| ZHANG Chun-ling,ZOU Yong,ZHANG Wan-hua,ZHOU Zong-qing,LI Chun-hua,JIANG Feng-ying.Sensitivity effect analysis of different highly pathogenic Porcine reproductive and respiratory syndrome virus samples on cells[J].Acta Agriculturae Zhejiangensis,2011,23(1):62. |
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| Authors: | ZHANG Chun-ling ZOU Yong ZHANG Wan-hua ZHOU Zong-qing LI Chun-hua JIANG Feng-ying |
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| Institution: | Animal Husbandry and Veterinary Research Institute, shanghai Academy of Agricultural Sciences, Shanghai 201106, China |
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| Abstract: | We collected 100 clinical samples from suspected Porcine reproductive and respiratory syndrome virus(PRRSV) infection occurred in pig farms for virus isolation.The positive samples of PRRSV detected by RT-PCR were divided into two groups by serum and tissue samples.PRRSV were isolated and cultured with Marc-145 and PAM cell.The results showed that 29 PRRSV,including 21 serum samples(72.5%) and 8 tissue samples(27.5%),were obtained from the 45 positive samples with cell culture.Nineteen and 24 PRRSV strains ... |
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| Keywords: | RRSV cell sensitivity analysis |
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