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| Bacillus subtilis Z-3菌株纤溶酶的分离纯化研究 | |
| 引用本文： | 袁洪水,张红丽,辛欣,朱宝成.Bacillus subtilis Z-3菌株纤溶酶的分离纯化研究[J].河北农业大学学报,2010,33(6). |
| 作者姓名： | 袁洪水 张红丽 辛欣 朱宝成 |
| 作者单位： | 1. 河北大学,药学院,河北,保定,071001;河北农业大学,生命科学学院,河北,保定,071001 2. 河北农业大学,生命科学学院,河北,保定,071001 3. 河北省保定市卫生监督所,河北,保定,071000 |
| 基金项目： | 河北省教育厅科研项目(Z2009123) |
| 摘 要： | 为获取微生物源溶栓药物,从土壤中获得产纤溶酶活性较高的1株枯草芽孢杆菌(Bacillus subtilis)Z-3。从Z-3菌株的发酵液中提取纤溶酶,利用DEAE-Sepharose Fast Flow阴离子交换层析和聚丙烯酰凝胶电泳等方法,对纤溶酶进行分离纯化。分离纯化得到1个单一组分(BSFE-1),SDS-PAGE电泳检测为单一条带,BSFE-1表观分子量为48 kDa,等电点为10.5,属丝氨酸蛋白酶类,纤维蛋白平板法检测纤溶酶活性BSFE-1的比活力为4 494.099 U/mg,BSFE-1在4~50℃和pH 4~11之间活性较稳定。 |
| 关 键 词： | 纤溶酶 分离纯化 等电点 酶学性质 |
Separation and purification of fibrinolytic enzyme from Bacillus subtilis Z-3 |
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| YUAN Hong-shui,ZHANG Hong-li,XIN Xin,ZHU Bao-cheng.Separation and purification of fibrinolytic enzyme from Bacillus subtilis Z-3[J].Journal of Agricultural University of Hebei,2010,33(6). | |
| Authors: | YUAN Hong-shui ZHANG Hong-li XIN Xin ZHU Bao-cheng |
| Institution: | YUAN Hong-shui1,2,ZHANG Hong-li2,XIN Xin3,ZHU Bao-cheng2(1.College of Pharmaceutical Science,Hebei University,Baoding 071001,China,2.College of Life Science,Agricultural University of Hebei,3.Baoding Health Inspection of Hebei,Baoding 071000,China) |
| Abstract: | The activity of a fibrinolytic enzyme produced from Bacillus subtilis Z-3 which was obtained from soil was examined by using fibrin plate method and its isoelectric point was determined via isoelectric focusing electrophoresis.By applying the DEAE-Sepharose Fast Flow anion exchange and polyacrylamide gel electrophoresis approaches,a single protein fraction(BSFE-1) with fibrinolytic activity was separated and purified from the culture of Bacillus subtilis Z-3.The SDS-PAGE result showed that BSFE-1 had only a... |
| Keywords: | fibrinolytic enzyme separation and purification isoelectric point enzymology character |
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