| siRNA干扰lZP3 mRNA表达的研究 |
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| 引用本文： | 庄淑珍,郭继娜,郑耀虎,李菁菁,张富春.siRNA干扰lZP3 mRNA表达的研究[J].中国农学通报,2007,23(6):23-23. |
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| 作者姓名： | 庄淑珍 郭继娜 郑耀虎 李菁菁 张富春 |
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| 作者单位： | 新疆大学生命科学与技术学院分子生物学重点实验室,乌鲁木齐,830046 |
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| 基金项目： | “国家自然科学基金资助项目”(No.30360062)，“中国博士后科学基金资助项目”(2005038619)，“新疆生物资源基因工程重点实验室开放课题资助项目”(XJDX0201-2006-03) |
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| 摘 要： | 【目的】为探索新疆草原兔尾鼠（Lagurus lagurus）卵透明带3（lZP3）在精卵结合中的机理，有效的控制草原兔尾鼠的生育；【方法】将RNA干扰载体pGenesil-ZP31和真核表达质粒p-ACLC一起通过尾静脉大容量快速注射法（Hydrodynamics-based transfection method, HD法）共注射小鼠。【结果】半定量RT-PCR和real-time PCR结果表明，2种质粒共注射时，pGenesil-ZP31的干涉作用至少发生在注射后的8 h到10 d之间；顺序性注射实验中，注射pGenesil-ZP31后间隔8 h和1 d再注射p-ACLC时，pGenesil-ZP31能在一定程度上干涉lZP3 mRNA的表达，间隔3 d以后lZP3 mRNA的表达几乎不受影响，未出现干涉现象。而注射p-ACLC后间隔1 h、2 h、4 h和8 h再注射pGenesil-ZP31，lZP3 mRNA的表达也受到了抑制，间隔24 h时抑制现象则消失；【结论】以载体为基础的体内RNAi过程具有时间依赖性，为在卵母细胞水平进一步研究siRNA对lZP3的干涉机制提供借鉴。
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| 关 键 词： | 野生蔬菜 野生蔬菜 研究与开发 学科 科学价值 |
| 修稿时间： | 2007-03-132007-03-23 |
Study on the lZP3 mRNA Expression after siRNA Interference |
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| Zhuang Shuzhen,Guo Jin,Zheng Yaohu,Li Jingjing,Zhang Fuchun.Study on the lZP3 mRNA Expression after siRNA Interference[J].Chinese Agricultural Science Bulletin,2007,23(6):23-23. |
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| Authors: | Zhuang Shuzhen Guo Jin Zheng Yaohu Li Jingjing Zhang Fuchun |
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| Institution: | .Key Laboratory of Molecular Biology, College of Life Science and Technology, Xinjiang University, Urumqi 830046 |
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| Abstract: | Objective]In order to explore lZP3 gene in the oocytes and sperms combination mechanism and control the population of the Lagurus lagurus.Method]The RNA interference vector pGenesil-ZP31 and the lZP3 expression vector were co-injected into the mice by Hydrodynamics-based transfection method (HD).Results] Results of semi-quantative RT-PCR and real-time PCR showed that, the interference effect was occurred between 8 h and 10 d when co-injected the 2 vectors. Study of sequential injections revealed that prior injection of pGenesil-ZP31 produced a significant suppression at 8 h and 1 d, which disappeared after 3 days. However, injection of p-ACLC followed by pGenesil-ZP31 at 1 h, 2 h, 4 h and 8 h, the expression of lZP3 mRNA was also suppression, which disappeared after 24 hours.Conclusion]Taken together, these results demonstrate that vector-based in vivo RNAi is a time-dependent process and offers the use for references in studying the mechanism of siRNA interference the Lagurus lagurus ZP3 at the oocytes level. |
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| Keywords: | Lagurus lagurus RNA interference vector Zona pellucida 3 Hydrodynamics-based transfection method |
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