| 罗非鱼源无乳链球菌S-核糖基高半胱氨酸酶基因（1uxS）的克隆及其推导蛋白的三维结构预测 |
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| 引用本文： | 马艳平,李嘉彬,郝乐,刘振兴,冯国清,周结珊,柯浩.罗非鱼源无乳链球菌S-核糖基高半胱氨酸酶基因（1uxS）的克隆及其推导蛋白的三维结构预测[J].南方水产,2013(1):43-47. |
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| 作者姓名： | 马艳平 李嘉彬 郝乐 刘振兴 冯国清 周结珊 柯浩 |
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| 作者单位： | 广东省农业科学院兽医研究所广东省兽医公共卫生公共实验室,广东广州510640 |
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| 基金项目： | 广东省农业科学院兽医研究所所长基金项目（2011SZJJ005）;广州市农业科技攻关项目（GZCQC1202FG04002-01） |
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| 摘 要： | 利用PCR技术对罗非鱼源无乳链球菌（Streptococcus agalactiae）S-核糖基高半胱氨酸酶（1uxS）基因全长DNA进行了扩增、克隆和序列测定，采用ExPAsy软件包预测了推导蛋白的特性，利用SwisS—Model服务器建立了luxS三维结构，利用Swiss—PDBviewer软件进行了蛋白质三维结构的分析。预测结果显示，罗非鱼源无乳链球菌luxS推导蛋白包括保守的酶活性中心和锌结合位点，具有影响生物被膜形成、毒力因子调控等特性功能；经拉氏构象图（Ramachandranplot）分析，所构建的z眦s的空间结构合理。
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| 关 键 词： | 罗非鱼 无乳链球菌 S-核糖基高半胱氨酸酶 三维结构 |
Cloning of Streptococcus agalactiae luxS gene from tilapia and 3D structure prediction of deduced protein |
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| MA Yanping,LI Jiabin,HAOLe,LIU Zhenxing,FENG Guoqing,ZHOU Jieshan,KE Hao.Cloning of Streptococcus agalactiae luxS gene from tilapia and 3D structure prediction of deduced protein[J].South China Fisheries Science,2013(1):43-47. |
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| Authors: | MA Yanping LI Jiabin HAOLe LIU Zhenxing FENG Guoqing ZHOU Jieshan KE Hao |
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| Institution: | ( Guangdong Public Lab. of Veterinary Public Health, Institute of Veterinary Medicine, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China) |
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| Abstract: | We have amplified, cloned and determined the sequence of Streptococcus agalactiae luxS gene from Tilapia sp. by PCR. The characteristics of the deduced luxS protein were predicted by ExPAsy software; the 3D structures of luxS and the deduced protein were established and analyzed by SWISS-Model and SwisS-PDBviewer software, respectively. The results indicate that the deduced luxS protein contains conserved active center and Zn2＋ binding site, which may affect biofilm formation and regulate virulence factor. The Ramachandram plot shows that the structure of modeled luxS protein is reasonable. |
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| Keywords: | Tilapia sp Streptococcus agalactiae luxS 3 D structure |
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