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麝香百合'雪皇后'多倍体诱导
引用本文:兰倩,杨利平.麝香百合'雪皇后'多倍体诱导[J].河北农业大学学报,2011,34(2):48-52.
作者姓名:兰倩  杨利平
作者单位:1. 河北农业大学园艺学院,河北保定,071001
2. 长江师范学院生命科学与技术学院,重庆,408100
摘    要:以麝香百合(Lilumlongiflorm)杂种系‘雪皇后'的试管苗为材料,在离体培养条件下,比较了秋水仙素附加2%二甲基亚砜(DMSO)对叶片和丛生苗的诱导作用效果.结果表明:叶片以0.05%的秋水仙素和2%DMSO处理72 h的诱变效果较好,四倍体率达到了10%.丛生苗以0.02%的秋水仙素和2%DMSO处理48 ...

关 键 词:百合  多倍体诱导  秋水仙素  形态学鉴定  细胞学鉴定

Polyploidy induction in Lilium longiflorum 'Snow Queen'
LAN Qian,YANG Li-ping.Polyploidy induction in Lilium longiflorum 'Snow Queen'[J].Journal of Agricultural University of Hebei,2011,34(2):48-52.
Authors:LAN Qian  YANG Li-ping
Institution:LAN Qian1,YANG Li-ping2(1.College of Horticulture,Agricultural University of Hebei,Baoding 071001,China,2.College of Life Science and Technology,Yangtze Normal University,Chongqing 408100,China)
Abstract:The seedlings of Lilium longiflorum hybrids 'Snow Queen' were used as materials.In vitro culture conditions,the mutagenic effects of different concentrations,different time of colchicine supplemented with 2% dimethyl sulfoxide(DMSO) to leaves and cluster of seedlings were compared.The results showed that the highest induction frequency(10%) was achieved with colchicine at 0.05% and DMSO at 2% for 72 h to the leaves,but to the cluster seedlings,the treatment with colchicine at 0.02% and DMSO at 2% for 48 h y...
Keywords:Lilium  poliploid induction  chochicine  morphological identification  cytological identification  
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