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新城疫病毒地方株分离鉴定及部分生物学特性研究
引用本文:许信刚,邢福珊,张耀相.新城疫病毒地方株分离鉴定及部分生物学特性研究[J].西北农业学报,2005,14(4):124-126.
作者姓名:许信刚  邢福珊  张耀相
作者单位:西北农林科技大学动物科技学院,陕西,杨凌,712100
基金项目:杨凌农业高新技术示范区科研基金资助项目(99KG9)。
摘    要:从某养鸡场发病率为90%、病死率为80%的鸡群中分离到一株病毒。通过血凝(HA)试验、血凝抑制 (H1)试验、鸡胚中和试验及回归试验初步鉴定为鸡新城疫病毒。继而进行了鸡胚半数感染量(EID50)、最小致 死量致死鸡胚平均死亡时间(MDT)、1日龄雏鸡脑内致病指数(ICPI)测定等生物学特性研究和对不同动物红 细胞的凝集试验,证明该分离株属新城疫强毒力毒株,命名为YL株。

关 键 词:新城疫病毒  强毒株  分离鉴定  生物学特性
文章编号:1004-1389(2005)04-0124-03
收稿时间:2004-12-10
修稿时间:2005-03-31

Isolation and Identification of Avian Virelent Newcastle Disease Virus and Its Biological Characteristics in Part
XU Xin-gang,XING Fu-shan and ZHANG Yao-xiang.Isolation and Identification of Avian Virelent Newcastle Disease Virus and Its Biological Characteristics in Part[J].Acta Agriculturae Boreali-occidentalis Sinica,2005,14(4):124-126.
Authors:XU Xin-gang  XING Fu-shan and ZHANG Yao-xiang
Institution:College of Animal Science and Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China;College of Animal Science and Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China;College of Animal Science and Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China
Abstract:One isolate virus was obtained from a chicken flock with the morbidity of 90% and mortality of 80%. The isolate strain can agglutinate chicken erythrocytes and this agglutination can be inhibited by Newcastle disease standard anti-serum. So it was conformed as NDV . Biological characteristics in part of NDV isolate strain were determinated including EID30, MDT, ICPI . According to the provided criterion, the results indicated that the avian NDV isolated strain is a virulent strain.
Keywords:Avian Newcastle disease  Virulent strain  Isolation and identification  Biological characteristics
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