| 条斑紫菜抗坏血酸过氧化酶基因的克隆及生物信息学分析 |
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| 引用本文： | 陈旸升,席海秀,佟少明,侯和胜.条斑紫菜抗坏血酸过氧化酶基因的克隆及生物信息学分析[J].天津农业科学,2014(4):8-10. |
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| 作者姓名： | 陈旸升 席海秀 佟少明 侯和胜 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁大连116081 |
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| 摘 要： | 结合电子克隆及RT-PCR技术获得条斑紫菜(Porphyrayezoensis)过氧化物酶基因。该基因cDNA序列长847 bp,包含编码245个氨基酸的开放阅读框。生物信息学分析结果显示,该基因编码蛋白无信号肽序列,与高等植物拟南芥的细胞质抗坏血酸过氧化物酶蛋白序列同源性较高,在细胞质中行使功能。此外,该基因的进化历程基本符合植物从低等到高等的进化过程。
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| 关 键 词： | 条斑紫菜 抗坏血酸过氧化物酶 克隆 生物信息学 |
Cloning and Bioinformatics Analysis of Ascorbate Peroxidase Gene in Porphyrayezoensis |
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| CHEN Yang-sheng,XI Hai-xiu,TONG Shao-ming,HOU He-sheng.Cloning and Bioinformatics Analysis of Ascorbate Peroxidase Gene in Porphyrayezoensis[J].Tianjin Agricultural Sciences,2014(4):8-10. |
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| Authors: | CHEN Yang-sheng XI Hai-xiu TONG Shao-ming HOU He-sheng |
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| Institution: | (School of Life Science, Liaoning Normal University, Dalian, Liaoning 116081, China) |
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| Abstract: | A novel gene of ascorbate peroxidase was cloned from Porphyrayezoensis, which with full-length of 847 bp and an open reading frame encoding 245 aa. The results of bioinformatics analysis showed that this protein sequence had no signal peptide and had highly homology to cytoplasm APX of higher plant Arabidopsis thaliana, implying PyAPX functioning in cytoplasm. Meanwhile, this gene evolved broadly in line with the evolution of plant from lower to higher. |
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| Keywords: | Porphyrayezoensis ascorbate peroxidase clone bioinformatic |
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