| 切花菊高效不定芽再生体系的建立 |
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| 引用本文： | 刘冰,杨际双,肖建忠,黄兆平,李单.切花菊高效不定芽再生体系的建立[J].河北农业大学学报,2011,34(1):25-29. |
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| 作者姓名： | 刘冰 杨际双 肖建忠 黄兆平 李单 |
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| 作者单位： | 1. 河北农业大学,园林与旅游学院,河北,保定,071001
2. 河北农业大学,园艺学院,河北,保定,071001 |
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| 基金项目： | 河北农业大学留学回国人员科研启动基金(2004-897) |
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| 摘 要： | 以"神马"和"出水芙蓉"2个切花菊品种的叶片为外植体,来建立不定芽再生体系.结果表明:基因型和植物生长调节剂是影响叶片不定芽再生的重要因素,并且较高浓度生长调节剂会导致不定芽和再生小苗玻璃化,不利于植株再生."神马"的最适直接诱导不定芽再生培养基为MS+NAA 1 mg/L+6-BA 1 mg/L,再生率高达955%;...
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| 关 键 词： | 切花菊 不定芽 再生 叶盘 |
Efficient shoots regeneration from adventitious buds of cutting chrysanthemum |
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| LIU Bing,YANG Ji-shuang,XIAO Jian-zhong,HUANG Zhao-ping,Li Dan.Efficient shoots regeneration from adventitious buds of cutting chrysanthemum[J].Journal of Agricultural University of Hebei,2011,34(1):25-29. |
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| Authors: | LIU Bing YANG Ji-shuang XIAO Jian-zhong HUANG Zhao-ping Li Dan |
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| Institution: | LIU Bing1,YANG Ji-shuang2,XIAO Jian-zhong1,HUANG Zhao-ping1,Li Dan1 (1.College of Landscape Architecture and Tourism,Agricultural University of Hebei,Baoding 071001,China,2.College of Horticulture,China) |
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| Abstract: | Using leaf disks of cutting chrysanthemum(Jinba and Chushui Furong)as explants,an efficient regeneration system from the adventitious buds was established.The results indicated that the genotypes and plant growth regulators greatly affected shoot regeneration from adventitious buds.High concentrations of the plant growth regulators increased the vitrification of adventitious buds and shoots from leaves,and then,decreased the shoot regeneration.The optimum medium which could directly induce shoot regeneratio... |
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| Keywords: | cutting chrysanthemum adventitious buds regeneration leaf discs |
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