| 高效液相色谱法测定茶叶中烟酸的含量 |
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| 引用本文： | 裴亭.高效液相色谱法测定茶叶中烟酸的含量[J].茶叶科学,2011,31(2):124-128. |
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| 作者姓名： | 裴亭 |
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| 作者单位： | 江苏出入境检验检疫局工业产品检测中心,江苏 南京 210001 |
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| 摘 要： | 利用高效液相色谱法单独对茶叶中常见的水溶性维生素—烟酸进行了测定。具体方法为：用Agilent 1100高效液相色谱系统,C18 4.6mm×150mm色谱柱,流动相：甲醇∶0.05mol/L磷酸二氢钾溶液（1∶9）,流速1.0mL/min,检测波长266nm。外标法测定茶叶中烟酸的含量,检测线性关系R2=0.9915,回收率为99.38%,RSD=2.18%（n=4）。研究表明,该方法操作简便,结果准确,具有较好的重现性与稳定性,可用于茶叶提取物中烟酸含量的测定。
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| 关 键 词： | 高效液相色谱 茶叶 烟酸 含量 |
| 收稿时间： | 2010-06-07 |
Determination of Nicotinic Acid in Tea Leaves by HPLC |
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| PEI Ting.Determination of Nicotinic Acid in Tea Leaves by HPLC[J].Journal of Tea Science,2011,31(2):124-128. |
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| Authors: | PEI Ting |
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| Institution: | Inspection Centre of Industry Product of JiangSu Entry-Exit Inspection and Quarantine Bureau ofThe People’s Republic of China, Nanjing 210001, China |
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| Abstract: | A method to measure nicotinic acid by high performance liquid chromatography was investigated in this work.Method: using Agilent 1100 HPLC series,C18 column(4.6mm×150 mm),mobile phase of MeOH∶0.05 mol/L KH2PO4(1∶9),with flow rate of 1.0 mL/min,and the detection wavelength at 266 nm.The nicotinic acid curve was linear with R2=0.9915.The average recovery rate for Nicotinic acid was 99.38% with RSD 2.18%(n=4).Conclusion: the method is simple and has good reproducibility.It coulde be used for analysis of nicotinic acid in tea leaves. |
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| Keywords: | HPLC tea leaves nicotinic acid content |
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