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| 巴西橡胶树中碱性转化酶HbNINa基因的克隆和表达模式分析 | |
| 引用本文： | 肖小虎,戚继艳,方永军,曹冰,龙翔宇,唐朝荣.巴西橡胶树中碱性转化酶HbNINa基因的克隆和表达模式分析[J].热带作物学报,2013,34(7):1264-1269. |
| 作者姓名： | 肖小虎 戚继艳 方永军 曹冰 龙翔宇 唐朝荣 |
| 作者单位： | 1. 海南大学农学院,海南海口 570228;中国热带农业科学院橡胶研究所,海南儋州 571737 2. 中国热带农业科学院橡胶研究所,海南儋州,571737 3. 海南大学农学院,海南海口,570228 |
| 基金项目： | 国家自然科学基金计划项目（No. 31170630）；海南省自然科学基金项目（No. 310094）； 国家863课题（No. 2013AA102605）。 |
| 摘 要： | 检索橡胶树EST和基因组数据库,发现了1个在叶片中高丰度表达的中碱性转化酶基因.采用RT-PCR和RACE技术获得了该基因的cDNA和基因组序列,命名为Hb NINa.序列分析结果显示,HbNINa基因cDNA序列的编码区(CDS)序列长度为1 719 bp,对应的基因组序列为3 696 bp.序列分析表明,该基因编码571个氨基酸多肽,推测其分子量大小为65.7 ku,等电点为6.36.HbNINa蛋白包含植物中碱性转化酶的全部保守结构域.基因组结构分析显示Hb NINa基因包含4个外显子和3个内含子.QPCR分析结果表明,HbNINa基因在在胶乳中表达水平极低,而在其他组织如树皮、树叶和雌花中的表达丰度相对较高.在叶片发育过程中,Hb NINa在叶片发育的初期高丰度表达,推测HbNINa可能参与叶片蔗糖利用,进而在橡胶树叶片发育过程中起重要作用. |
| 关 键 词： | 巴西橡胶树 中碱性转化酶 基因结构 表达分析 叶片发育 |
Cloning and Expression Analysis of an Alkaline/Neutral Invertase Gene in Hevea brasliensis |
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| XIAO Xiaohu,QI Jiyan,FANG Yongjun,CAO Bing,LONG Xiangyu and TANG Chaorong.Cloning and Expression Analysis of an Alkaline/Neutral Invertase Gene in Hevea brasliensis[J].Chinese Journal of Tropical Crops,2013,34(7):1264-1269. | |
| Authors: | XIAO Xiaohu QI Jiyan FANG Yongjun CAO Bing LONG Xiangyu and TANG Chaorong |
| Abstract: | Based on the Hevea EST and genome sequence database, we cloned one alkaline/neutral invertase gene, HbNINa, which was highly expressed in the leaf of Hevea brasiliensis. Sequence analysis revealed that HbNINa coding sequence was 1 719 bp, and the deduced peptide was 571 amino acids in length, with an average molecular weight of 65.7 ku and a pIvalue of 6.36. The HbNINa protein contained all the conserved domains of typical plant alkaline/neutral invertases. Exon/intron structure analysis showed that the HbNINa gene contained four exons and three introns. The real-time RT-PCR results indicated that the HbNINa expression was mainly found in the bark, leaf and female flower, whereas barely detectable in the latex. HbNINa was also found highly expressed in bronze-stage leaf and down-regulated with leaf maturation, suggesting the important roles of this gene in the process of leaf maturation in rubber tree. |
| Keywords: | Hevea brasiliensis Alkaline/neutral invertase Gene structure Expression analysis Leaf development |
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