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| 根癌农杆菌介导转化获得耐逆性增强的高羊茅转基因植株 | |
| 引用本文： | 吴关庭,陈锦清,胡张华,郎春秀,陈笑芸,王伏林,金卫,夏英武.根癌农杆菌介导转化获得耐逆性增强的高羊茅转基因植株[J].中国农业科学,2005,38(12):2395-2402. |
| 作者姓名： | 吴关庭 陈锦清 胡张华 郎春秀 陈笑芸 王伏林 金卫 夏英武 |
| 作者单位： | 1. 浙江省农业科学院病毒学与生物技术研究所,杭州,310021;浙江大学原子核农业科学研究所,杭州,310029 2. 浙江省农业科学院病毒学与生物技术研究所,杭州,310021 3. 浙江大学原子核农业科学研究所,杭州,310029 |
| 基金项目： | 浙江省“新世界151人才基金”资助项目和浙江绿洲生态股份有限公司风险投资项目 |
| 摘 要： | 为改良草坪型高羊茅（Festuca arundinacea Schreb.）的耐逆性，以成熟种子来源的胚性愈伤组织为受体材料，通过农杆菌介导法将拟南芥（Arabidopsis thaliana）耐逆相关CBF1基因导入4个供试品种的基因组，经GUS染色、PCR检测和Southern杂交分析验证，获得了112株转基因植株，转化频率为0.92% ~2.87%，不同品种间存在差异。试验表明，在高盐与高渗胁迫下，转基因植株具有显著生长优势，存活率极显著高于非转化对照植株，经低温、高温、干旱和高盐等逆境胁迫处理后的叶片相对电导率平均较对照植株低25% ~ 30%，证明转基因植株的耐逆性有所增强。考察发现，非胁迫条件下CBF1基因的组成型超表达使转基因植株的生长受到抑制。 |
| 关 键 词： | 高羊茅 根癌农杆菌 遗传转化 CBF1基因 耐逆性 |
| 收稿时间： | 03 4 2005 12:00AM |
| 修稿时间： | 2005-03-04 |
Production of Transgenic Tall Fescue Plants with Enhanced Stress Tolerance by Agrobacterium tumefaciens-Mediated Transformation |
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| WU Guan-ting,CHEN Jin-qing,HU Zhang-hua,LANG Chun-xiu,CHEN Xiao-yun,WANG Fu-lin,JIN Wei,XIA Ying-wu.Production of Transgenic Tall Fescue Plants with Enhanced Stress Tolerance by Agrobacterium tumefaciens-Mediated Transformation[J].Scientia Agricultura Sinica,2005,38(12):2395-2402. | |
| Authors: | WU Guan-ting CHEN Jin-qing HU Zhang-hua LANG Chun-xiu CHEN Xiao-yun WANG Fu-lin JIN Wei XIA Ying-wu |
| Institution: | 1. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021; 2. Institute of Nuclear-Agricuhural Sciences, Zhejiang University, Hangzhou 310029 |
| Abstract: | In order to improve stress tolerance of turf-type tall fescue (Festuca arundinacea Schreb.), Agrobacterium tumefaciens strain EHA105 carrying plasmid pCMD containing stress tolerance-related CBF1 gene from Arabidopsis thaliana was used to transform mature seeds-derived embryogenic calli of four tested cultivars. One hundred and twelve transgenic plants from thirty-two independent lines were regenerated, which were confirmed by histochemical detection of GUS activity, PCR assay and Southern hybridization analysis. The transformation frequency ranged from 0.92% to 2.87% with apparent differences among the cultivars. Stress tolerance of transgenic plants were enhanced, which was testified by the facts that the transgenic plants had distinct growth superiority and significantly higher survival rate than non-transformed ones under high salt and high osmotic stresses, and that the relative electronic conductivity of in vitro leaves treated with low and high temperatures, dehydration and high salt stresses was 25%-30% lower in transgenic plants than in control plant. In addition, it was observed that growth of transgenic plants was inhibited due to constituent overexpression of CBF1 gene under normal environmental conditions. |
| Keywords: | Tall fescue (Festuca arundinacea Schreb ) Agrobacterium tumefaciens Genetic transformation CBF1 gene Stress tolerance |
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