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| 嗜温气单胞菌的鉴定及其外膜蛋白型研究 | |
| 引用本文： | 胡秀彩,郑璐,吕爱军,蒋继宏.嗜温气单胞菌的鉴定及其外膜蛋白型研究[J].中国动物检疫,2009,26(1):31-32. |
| 作者姓名： | 胡秀彩 郑璐 吕爱军 蒋继宏 |
| 作者单位： | 1. 徐州师范大学生命科学学院,江苏,徐州,221116;江苏省药用植物生物技术重点实验室,江苏,徐州,221116 2. 徐州师范大学生命科学学院,江苏,徐州,221116 3. 江苏省药用植物生物技术重点实验室,江苏,徐州,221116 |
| 基金项目： | 国家自然科学基金，江苏省高校自然科学研究计划，徐州师范大学自然科学基金 |
| 摘 要： | 从病鳖、鳗鱼体内分离到2株嗜温气单胞菌(TAh-3、EAs-1),经形态学观察、生理生化特性等鉴定,确定TAh-3为嗜水气单胞菌,EAs-1为温和气单胞菌。动物感染试验结果表明,TAh-3株为致病性嗜水气单胞菌,对鲫鱼的致死率100%,EAs-1株为非致病性温和气单胞菌。SDS-PAGE电泳结果显示,TAh-3株外膜蛋白(OMPs)型由7条蛋白带组成,其中1条主带大小为40.0kDa;EAs-1株OMPs型由8条蛋白带组成,其中2条主带大小为38.0kDa、45.0kDa。 |
| 关 键 词： | 嗜水气单胞菌 温和气单胞菌 鉴定 外膜蛋白型 |
Identification of Aeromonas and Their Outer Membrane Protein Patterns |
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| Hu Xiucai,Zheng Lu,Lv Aijun,Jiang Jihong.Identification of Aeromonas and Their Outer Membrane Protein Patterns[J].China Journal Of Animal Quarantine,2009,26(1):31-32. | |
| Authors: | Hu Xiucai Zheng Lu Lv Aijun Jiang Jihong |
| Institution: | ,(School of life sciences,Xuzhou normal university,Xuzhou221116,China) |
| Abstract: | Two bacterial strains were isolated from soft-shelled turtles and eel fishes,and tested for morpholohic,physiological and biochemistric characteristics,and the strain TAh-3 was classified as Aeromonas hydrophila,while EAs-1 was classified as Aeromonas sobria.The results of pathogenicity test on crucian carp and mice showed that TAh-3 strain are pathogenic Aeromonas hydrophila,resulting in 100% lethal rate in crucian carp,and EAs-1 strain was nonpathogenic Aeromonas sobria.Moreover,the outer membrane proteins(OMPs) of Aeromonas showed that TAh-3 OMPs pattern was composed of 7 protein bands,which had a major band with the molecular weight of 40.0 kDa;while EAs-1 OMPs pattern was composed of 8 protein bands,which had the two major bands such as 38.0 kDa and 45.0 kDa. |
| Keywords: | Aeromonas hydrophila Aeromonas sobria Identification outer membrane proteins |
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