Somatic hybridization between Solanum tuberosum and species of the S. nigrum complex: Selection of vigorously growing and flowering plants |
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| Authors: | K Horsman JEM Bergervoet E Jacobsen |
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| Institution: | (1) The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, P.O. Box 386, 6700 Aj Wageningen, the Netherlands |
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| Abstract: | Fusion experiments were performed between diploid (2n = 2x = 24) or tetraploid (2n = 4x = 48) potato genotypes and four species
of the Solanum nigrum complex, namely S. nigrum (2n = 6x = 72), S. villosum (2n = 4x = 48), S. chenopodioides (2n = 2x = 24)
or S. americanum (2n = 2x = 24 and 2n = 6x = 72). All five accessions of the S. nigrum-species were successfully hybridized
with at least one of the potato genotypes. Somatic combining abilities were influenced by the ploidy level as well as the
genotype of the parental species. The use of kanamycin or hygromycin resistance as cell-selectable markersystem had no influence
on somatic combining ability, but such markers can be useful to improve efficient selection of somatic hybrids in sufficient
numbers. At least 20% of the hybrids of each successful combination performed well in vitro. However, only 60 genotypes out
of 761 somatic hybrids were vigorous as well as flowering in the greenhouse. Analysis of the DNA content of somatic hybrids
could be used as a criterion for the indirect selection in vitro of hybrids that were vigorous in the greenhouse. Flowering
somatic hybrids of S. nigrum (+) 2x potato and S. americanum (+) 4x potato were selected with the aim of introgression of
resistance traits after recurrent backcrossing with cultivated potato.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | potato Solanum nigrum complex somatic hybridization hybrid selection criterions cell-selectable markers DNA content |
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