| 新疆雪莲芽分化过程中抗氧化酶体系活性的变化 |
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| 引用本文： | 徐春明,赵兵.新疆雪莲芽分化过程中抗氧化酶体系活性的变化[J].安徽农业科学,2009,37(9). |
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| 作者姓名： | 徐春明 赵兵 |
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| 作者单位： | 北京工商大学化学与环境工程学院,北京市植物资源研究开发重点实验室,北京,100048;中国科学院过程工程研究所,生化工程国家重点实验室,北京,100080 |
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| 摘 要： | 目的]为新疆雪莲的高频再生提供理论指导。方法]以雪莲胚性愈伤组织为材料,在pH值为5.85~5.90的MS+2.0 mg/L NAA+0.5 mg/L 6-BA+0.5%琼脂培养基中进行继代培养,研究了培养过程中超氧化物酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的变化。结果]新疆雪莲芽分化过程中胚性愈伤组织和非胚性愈伤组织的SOD活性逐渐增加,在培养24 h时达到最大。胚性愈伤组织的POD活性都大于非胚性愈伤组织;非胚性愈伤组织POD活性在培养过程中呈缓慢上升的趋势,而胚性愈伤组织POD活性呈双峰曲线变化。CAT在胚性愈伤组织培养前期活性较低。进入芽分化过程后,其活性逐渐升高。结论]新疆雪莲芽分化过程中,SOD与CAT活性变化趋势基本一致,与POD的活性变化不同。
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| 关 键 词： | 新疆雪莲 芽分化 抗氧化酶体系 |
Activity Change of Antioxidase System in Bud Differentiation Process of Saussurea involucrata |
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| XU Chun-ming et al.Activity Change of Antioxidase System in Bud Differentiation Process of Saussurea involucrata[J].Journal of Anhui Agricultural Sciences,2009,37(9). |
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| Authors: | XU Chun-ming |
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| Institution: | XU Chun-ming et al(Beijing Key Laboratory of Plant Resources Research , Development,College of Chemical , Environmental Engineering,Beijing Technology , Business University,Beijing 100037) |
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| Abstract: | Objective] The study was to provide the theoretical guidance for the regeneration of Saussurea involucrate with high frequency.Method]With the embryogenic callus of S.involucrata as the material,they were subculture in medium of MS+2.0 mg/L NAA+0.5 mg/L 6-BA+0.5% agar with pH value of 5.85-5.90.In culture process,the changes of activity of SOD,POD and CAT were studied.Result]In bud differentiation process of S.involucrata,the SOD activities of non embryogenic callus and embryogenic callus were increased ... |
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| Keywords: | Saussurea involucrata Bud differentiation Antioxidase system |
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