| 斑点免疫金渗滤试验检测绵羊脑多头蚴病的研究 |
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| 引用本文： | 王春仁,于万才,仇建华,许腊梅,于克江,李洪彬,徐梅倩,何国声,顾越星,曹杰,朱顺海.斑点免疫金渗滤试验检测绵羊脑多头蚴病的研究[J].中国兽医寄生虫病,2001,9(1):12-14. |
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| 作者姓名： | 王春仁 于万才 仇建华 许腊梅 于克江 李洪彬 徐梅倩 何国声 顾越星 曹杰 朱顺海 |
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| 作者单位： | 1. 黑龙江省兽医科学研究所富裕161200
2. 中国农科院上海家畜寄生虫病研究所上海200232 |
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| 摘 要： | 为了寻找一种快速、简便诊断绵羊脑多头蚴病的新方法，应用自行配制的胶体金探针标记SPA，建立了斑点免疫金渗滤试验（DIGFA）。该法同ELISA具有良好的相符性。将抗原、待检血清滴加于膜上进行反应，数分钟内即可用肉眼进行结果判定。本法具有操作简单、反应快速、敏感性强、重复性好、不需要贵重仪器等优点，适合于基层推广应用。
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| 关 键 词： | 斑点金渗滤法 脑多头蚴病 绵羊 诊断 |
| 文章编号： | 1005-0868(2001)01-0012-03 |
| 修稿时间： | 2000年6月12日 |
THE APPLICATION OF DOT-IMMUNOGOLD FILTRATION ASSAY FOR THE DETECTION OF COENUROSIS IN SHEEP |
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| WANG Chun-ren,YU Wan-cai,QIU Jian-hua,XU La-mei,YU Ke-jiang,LI Hong-bin,XU Mei-qian,HE Guo-sheng,GU Yue-xing,CAO Jie,ZHU Shu-hai.THE APPLICATION OF DOT-IMMUNOGOLD FILTRATION ASSAY FOR THE DETECTION OF COENUROSIS IN SHEEP[J].Chinese Journal of Veterinary Parasitology,2001,9(1):12-14. |
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| Authors: | WANG Chun-ren YU Wan-cai QIU Jian-hua XU La-mei YU Ke-jiang LI Hong-bin XU Mei-qian HE Guo-sheng GU Yue-xing CAO Jie ZHU Shu-hai |
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| Institution: | WANG Chun-ren1,YU Wan-cai1,QIU Jian-hua1,XU La-mei1,YU Ke-jiang1,LI Hong-bin1,XU Mei-qian2,HE Guo-sheng2,GU Yue-xing2,CAO Jie2,ZHU Shu-hai2 |
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| Abstract: | In order to estiblish a new method of detecting coenurosis in sheep, The dot-immunogold Filtrate Assay (DIGFA) by labeled with colloidal gold were established for the detection of coenurus cerebralis antibody. The method had a good agreement with ELISA, The antigen-antibody reaction took place on the membrane during the filtration process, the experiment result could be read with naked eyes within a few minutes. It was proved this method is quite simple, reacts quickly, sensitivity, so it can be used in field. |
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| Keywords: | DIGFA Cornurosis cerebralis Sh |
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