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| 猪等孢球虫PCR诊断方法的建立 | |
| 引用本文： | 刘光辉,宁长申,张龙现,程俊贞,菅复春.猪等孢球虫PCR诊断方法的建立[J].中国农学通报,2007,23(9):3-3. |
| 作者姓名： | 刘光辉 宁长申 张龙现 程俊贞 菅复春 |
| 作者单位： | 1. 河南省动物疫病预防控制中心,河南,郑州,450008 2. 河南农业大学牧医工程学院,河南郑州,450002 3. 许昌市畜牧兽医工作站,河南许昌,461000 |
| 基金项目： | 河南省自然科学基金资助项目（0311032400）. |
| 摘 要： | 参照GenBank收录的Isospora suis 18SrRNA基因全序列(U97523),利用引物分析软件Oligo 6.57和Primer Premier 5.0设计了一对引物(上游引物:5'-tcctgcgagtactcatatgc-3';下游引物:5'-gttcagc-tacgcataccttg-3'),首次扩增出猪等孢球虫分离株的18SrRNA基因序列,结合GenBank上登录的相关原虫序列,用DNAstar4.0软件比较其同源性,经Clustalx1.81序列比对,Paup4.0、Treeview3.0、Phylip种系发育关系软件分析后,证实该分离株为猪等孢球虫,并且河南不同地区之间的猪等孢球虫没有明显遗传差异。 |
| 关 键 词： | 猪等孢球虫 PCR 18SrRNA基因 种系发育关系 |
| 修稿时间： | 2007-04-09 |
Development of PCR for Diagnosing Isospora Suis |
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| Liu Guanghui,Ning Changshen,Zhang Longxian,Cheng Junzhen,Jian Fuchun.Development of PCR for Diagnosing Isospora Suis[J].Chinese Agricultural Science Bulletin,2007,23(9):3-3. | |
| Authors: | Liu Guanghui Ning Changshen Zhang Longxian Cheng Junzhen Jian Fuchun |
| Institution: | Henan Provincial Center for Animal Disease Control and Prevention, Zhengzhou 450008;2College of Animal Husbandry and Veterinary medicine, Henan Agricaltural University, Zhengzhou 450002; Pasturage Veterinary Station of Xuchang City, Xuchang 461000 |
| Abstract: | One pair of primer(P1:5'-tcctgcgagtactcatatgc-3';P2:5'-gttcagctacgcataccttg-3')was designed by using Oligo 6.57,Primer Premier 5.0 according to the sequence published by the GenBank.18SrRNA gene of I.suis which isolated from different area in Henan Province was amplifed succesfully for the first time by the primer.The result was analysis by Paup and Phylip revealed that the homology of I.suis isolated from different area in Henan Province was 100%,and there was no variation consisted in Henan Isospora suis. |
| Keywords: | Isospora suis PCR 18SrRNA gene Phylogenetic development |
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