| 舞毒蛾核型多角体病毒室内增殖的研究 |
| |
| 引用本文： | 梁洪柱,陈倩,田会鹏,梁晓梅.舞毒蛾核型多角体病毒室内增殖的研究[J].林业科学研究,2013,26(5):593-597. |
| |
| 作者姓名： | 梁洪柱 陈倩 田会鹏 梁晓梅 |
| |
| 作者单位： | 北京市西山试验林场,北京,100093 |
| |
| 摘 要： | 试验研究了饲毒龄期、饲毒浓度对舞毒蛾存活、幼虫体质量、虫尸质量和含毒量的影响以及饲毒时间对舞毒蛾含毒量的影响.结果表明:饲毒龄期和饲毒浓度对幼虫存活率和体质量增长均有不同程度的影响,饲毒龄期越小、饲毒浓度越高,死亡时间越早,死亡率越高,体质量增长越缓慢;而且饲毒时龄期越小、饲毒浓度越高,虫尸质量也越小,其含毒量也越低.饲毒时间与含毒量成抛物线型,饲毒时间过短或过长,体内含毒量均较低.由于5龄幼虫饲毒后有78％以上个体发育至蛹期,不发生死亡现象,因此,室内增殖舞毒蛾核型多角体病毒时,应选取4龄初幼虫喂饲1.0×106 PIB·mL-1病毒为宜,接毒10d后开始收集饲毒幼虫.
|
| 关 键 词： | 舞毒蛾 舞毒蛾核型多角体病毒 增殖 |
| 收稿时间： | 2013/3/15 0:00:00 |
Study on the Mass Propagation of Lymantria dispar Nuclear Polyhedrosis Virus (LdNPV) and Its Application |
| |
| LIANG Hong-zhu,CHEN Qian,TIAN Hui-peng and LIANG Xiao-mei.Study on the Mass Propagation of Lymantria dispar Nuclear Polyhedrosis Virus (LdNPV) and Its Application[J].Forest Research,2013,26(5):593-597. |
| |
| Authors: | LIANG Hong-zhu CHEN Qian TIAN Hui-peng and LIANG Xiao-mei |
| |
| Institution: | Beijing Experimental Forest Farm, Beijing 100093, China;Beijing Experimental Forest Farm, Beijing 100093, China;Beijing Experimental Forest Farm, Beijing 100093, China;Beijing Experimental Forest Farm, Beijing 100093, China |
| |
| Abstract: | The influence of instar of the host larvae and the concentration of Lymantria dispar nuclear polyhedrosis virus (LdNPV) on larvae survival rate, larvae mass, dead body mass and the mean number of LdNPV per larval of dead body were studied. And also, the effect of feeding time on the toxicity of LdNPV was studied. The results indicated that the influence of host larvae and the concentration of LdNPV on the survival rate and larvae mass were significantly different among individuals. The younger the instar of the host larvae, the higher the concentration of LdNPV, the earlier the dead time, the higher the mortality rate, the slower the body mass' increase and the lower the toxicity. The relationship between the feeding time and the concentration of LdNPV followed a parabolic curve, i.e. both the shorter or longer time resulted in lower LdNPV concentration. It was proved that the 2nd or the 5th larvae after feeding with LdNPV (78%) were not suitable for LdNPV production, so the authors believe that the 4th instar larval fed with virus at the 1.0×106 PIB·mL-1 concentration for 10days is the most suitable condition for the mass production of LdNPV in laboratory. |
| |
| Keywords: | Lymantria dispar Lymantria dispar nuclear polyhedrosis virus(LdNPV) mass propagation |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《林业科学研究》浏览原始摘要信息 |
| 点击此处可从《林业科学研究》下载免费的PDF全文 |
|
