| 莴苣叶斑病菌的分离鉴定及弱毒菌株致病力的衰退 |
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| 引用本文： | 潘显婷,陆训,庞茜丹,高必达,周倩.莴苣叶斑病菌的分离鉴定及弱毒菌株致病力的衰退[J].湖南农业大学学报(自然科学版),2016,42(5):538-542. |
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| 作者姓名： | 潘显婷 陆训 庞茜丹 高必达 周倩 |
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| 作者单位： | 湖南农业大学植物保护学院，湖南长沙 410128; 植物病虫害生物学与防控湖南省重点实验室，湖南长沙 410128 |
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| 基金项目： | 湖南省大学生科技创新项目(XCX16080) |
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| 摘 要： | 利用组织分离法,从感染叶斑病的莴苣叶片上分离得到4株真菌,ITS序列分析鉴定为匍柄霉(Stemphylium spp.)。科赫氏法则验证其致病性时发现,菌株WS–01较其他菌株致病力减弱,且生长缓慢。用纤维素吸附法提取菌株WS–01的dsRNA,发现WS–01含有3条1~5 kb的dsRNA条带,提示WS–01菌株内含有dsRNA病毒。利用高温–利巴韦林–尖端脱毒结合的方法消除WS–01内的病毒,所得菌株WS–01–D与菌株WS–01相比,致病性增强且表型恢复正常,提示弱毒菌株WS–01的致病力减弱与其所含dsRNA有关。
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| 关 键 词： | 匍柄霉属病菌 弱毒菌株 dsRNA病毒 病毒消除 |
Isolation of the pathogens causing leaf spot of lettuce and analysis of the reason causing the attenuated virulence |
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| Abstract: | Fourstrains were isolated from lettuce with leaf spot disease by tissue isolation method and identified as Stemphyliumspp. with ITS sequences analysis. An attenuated strain with debilitated pathogenicity and slower growth rate named WS–01 was found by artifical inoculation. The cellulose CF11 method was used to extract dsRNA to determine the relation between the debilitated pathogenicity and its dsRNA mycovirus, three dsRNAs ranged from 1 kb to 5 kb were detected in the WS–01 strain. High temperature-ribavirin-hyphal tip isolation method for elimination of virus was conducted. The results showed that the pathogenicity of mycovirus-eliminated strain WS–01–D was enhanced and the phenotype returned to normal compared to the original strain, indicating the abnormal phenotype of strain WS–01 was related to its dsRNA. |
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| Keywords: | Stemphylium attenuated strain dsRNA mycovirus virus elimination |
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