| 人胎盘TRAIL基因的cDNA克隆及序列测定 |
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| 引用本文： | 张明烽,唐 爽,宋红卫.人胎盘TRAIL基因的cDNA克隆及序列测定[J].西北农林科技大学学报(社会科学版),2005,33(2):6-8,13. |
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| 作者姓名： | 张明烽 唐 爽 宋红卫 |
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| 作者单位： | 西北农林科技大学,生物工程研究所,陕西,杨凌,712100 |
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| 基金项目： | 国家"863"高技术资助项目(2001AA213081) |
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| 摘 要： | 根据GeneBank(U37518)中TRAIL(肿瘤坏死因子相关的凋亡诱导配体)的cDNA序列,设计扩增引物。采用RT-PCR从人胎盘中扩增出TRAIL基因的全长cDNA。产物纯化后连至pGEM-TEasy载体,转化大肠杆菌DH5α。筛选阳性克隆菌,酶切鉴定并进行序列测定。结果表明,本试验成功地克隆了TRAIL基因的1039bp全长cDNA。
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| 关 键 词： | 肿瘤坏死因子相关的凋亡诱导配体(TRAIL) RT-PCR 序列测定 人胎盘 |
| 文章编号： | 1671-9387(2005)02-0006-03 |
| 收稿时间： | 3/3/2004 12:00:00 AM |
Cloning and sequencing of cDNA of TRAIL from human placenta |
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| ZHANG Ming-feng,TANG Shuang,SONG Hong-wei,ZHANG Yong.Cloning and sequencing of cDNA of TRAIL from human placenta[J].Journal of Northwest Sci-Tech Univ of Agr and,2005,33(2):6-8,13. |
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| Authors: | ZHANG Ming-feng TANG Shuang SONG Hong-wei ZHANG Yong |
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| Institution: | (Institute of Bio-Engineering,Northwest A & F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | The financial risk of universities and colleges is produced with the obtainment of legal person position and expansion of independence as well as the change of educational structure and scales. The three main risks are unbalanced risk of financial situation, debt risk and risk caused by industries run by universities and colleges. The causes of each risk are different, and the precautions mechanism should be established respectively. |
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| Keywords: | TNF-related apoptosis-inducing ligand (TRAIL) RT-PCR sequencing human placenta |
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