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| 芽孢杆菌HSY-8-1对植物病原真菌的抑制及其抑菌产物特性 | |
| 引用本文： | 郭金鹏,刘晓昌,仝赞华,郭荣君,潘洪玉.芽孢杆菌HSY-8-1对植物病原真菌的抑制及其抑菌产物特性[J].吉林农业大学学报,2010,32(1):29-33. |
| 作者姓名： | 郭金鹏 刘晓昌 仝赞华 郭荣君 潘洪玉 |
| 作者单位： | 1. 解放军疾病预防控制所,北京,100166 2. 吉林大学农学部植物学院,长春,130062 3. 中国农业科学院植物保护研究所,北京,100081 |
| 基金项目： | “十一五”国家科技支撑计划项目(2006BAD08A08);;长春市国际合作项目(06GH07);;农业部生物防治重点实验室基金项目(LOBCR-01-15) |
| 摘 要： | 为挖掘用于生物防治的微生物资源,自海南蚕豆枯萎病区土壤分离1株芽孢杆菌HSY-8-1,经抗菌试验分析表明,其代谢产生的拮抗物质对引发植物立枯病和根腐病的立枯丝核菌(Rhizoctonia solani)和镰刀菌(Fusariumspp.)抑菌作用较强,具有使病菌菌丝扭曲、缢缩等致畸效应,并抑制立枯丝核菌的菌核形成。研究表明,在NB液体培养基中发酵时,进入稳定生长期后开始产生抑菌物质,72 h时累积达到最大值。这种抑菌物质的特性为外泌型、水溶性,可透过0.22μm的微孔滤膜,对热、酸、碱、蛋白酶水解、紫外线照射等影响有较强的稳定性。 |
| 关 键 词： | 芽孢杆菌HSY 8 1 抑菌物质 立枯丝核菌 镰刀菌 |
| 收稿时间： | 2008-11-14 |
| 修稿时间： | 2009-03-11 |
Antagonism of Bacillus sp. HSY-8-1 Against Plant Pathogens and Properties of Its Anti-Fungal Substance |
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| GUO Jin-peng,LIU Xiao-chang,TOG Zan-hua,GUO Rong-jun,PA Hong-yu.Antagonism of Bacillus sp. HSY-8-1 Against Plant Pathogens and Properties of Its Anti-Fungal Substance[J].Journal of Jilin Agricultural University,2010,32(1):29-33. | |
| Authors: | GUO Jin-peng LIU Xiao-chang TOG Zan-hua GUO Rong-jun PA Hong-yu |
| Institution: | 1.Institute of Disease Prevention and Control, Beijing 100166, China; 2. College of Plant Science, Agronomy Division of Jilin University, Changchun 130062, China; 3. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081,China |
| Abstract: | In order to excavate microbial resources for bio-control use, Bacillus sp.HSY-8-1 was isolated from broadbean field samples collected in Hainan.HSY8-1 and its anti-fungal substance can effectively inhibit Rhizoctonia solani and Fusarium spp.growth.They cause hyphae of Rhizoctonia solani and Fusarium spp.malformation and delay sclerotia of Rhizoctonia solani germination.The study demonstrated that when fermented in NB liquid medium and after the growth entering into the stable phase,it began to produce antif... |
| Keywords: | Bacillus sp HSY-8-1 anti-fungal substance Rhizoctonia solani Fusarium spp |
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