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Diagnosis of dehydratase inhibitors in melanin biosynthesis inhibitor (MBI-D) resistance by primer-introduced restriction enzyme analysis in scytalone dehydratase gene of Magnaporthe grisea |
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| Authors: | Kaku Koichiro Takagaki Makiichi Shimizu Tsutomu Nagayama Kozo |
| Institution: | Kumiai Chemical Industry Co Ltd, Life Science Research Institute, 3360 Kamo, Kikugawa, 439-0031 Japan. k-kaku@kumiai-chem.co.jp |
| Abstract: | We have established a simple diagnosis method for rice blast fungus resistant to MBI-D. This involves the preparation of PCR templates directly from the lesions in combination with primer-introduced restriction enzyme analysis PCR (PIRA-PCR). |
| Keywords: | carpropamid scytalone dehydratase MBI‐D fungicide resistance point mutation primer‐introduced restriction enzyme analysis |
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