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巴氏醋酸杆菌As1.41培养基的优化研究
引用本文:申秋华,白文杰.巴氏醋酸杆菌As1.41培养基的优化研究[J].安徽农业科学,2012(36):17455-17456,17459.
作者姓名:申秋华  白文杰
作者单位:海军工程大学勤务学院,天津,300450;海军工程大学勤务学院,天津,300450
摘    要:目的]为了对巴氏醋酸杆菌As1.41培养基进行优化。方法]以巴氏醋酸杆菌As1.41为出发菌株,采用酸碱滴定法检测发酵液中醋酸含量。通过单因子试验和正交设计,对该菌株产酸发酵培养基进行优化。结果]巴氏醋酸杆菌As1.41的最佳发酵培养基最佳碳源和氮源分别是葡萄糖和酵母膏;巴氏醋酸杆菌As1.41的最佳发酵培养基配方为:葡萄糖1%,酵母膏1%,乙醇4%,乙酸0%,此条件下醋酸产量可达56.61g/L。结论]该研究可为醋酸发酵的工业化生产提供科学依据。

关 键 词:巴氏醋酸杆菌As1.41  优化  培养基

Study on Optimized Fermentation Medium of Acetic Acid Bacteria As1.41
Institution:SHEN Qiu-hua et al(College of Logistics,Naval University of Engineering,Tianjin 300450)
Abstract:Objective] The research aimed to optimize the medium of acetic acid bacteria As1.41.Method] Acetic acid bacteria As1.41 was taken as the experimental spawn,and the acid-base titration was adopted to measure acetic acid contents.The optimizing of the medium used in the production of acetic acid was studied by single factor and the orthogonal experiments.Result] The best carbon and nitrogen sources were glucose and yeast extract.The best proportion of the medium contents for Asl.41 was glucose 1%,yeast extract 1%,ethanol 4%,and acetic acid 0%.Under this condition,the output of acetic acid could reach 56.6 g/L.Conclusion] The study could provide reference for large-scale production of acetic acid fermentation.
Keywords:Acetic acid bacteria As1  41  Optimization  Medium
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