| 基于线粒体Cyt b基因的黄鳍马面鲀种群分析 |
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| 引用本文： | 李玉芳,李永振,陈国宝,孙典荣,于杰,崔科.基于线粒体Cyt b基因的黄鳍马面鲀种群分析[J].水产学报,2014,38(1):23-32. |
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| 作者姓名： | 李玉芳 李永振 陈国宝 孙典荣 于杰 崔科 |
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| 作者单位： | 中国水产科学研究院南海水产研究所, 广东 广州 510300;上海海洋大学水产与生命学院, 上海 201306;农业部南海区渔政局, 广东 广州 510080;中国水产科学研究院南海水产研究所, 广东 广州 510300;中国水产科学研究院南海水产研究所, 广东 广州 510300;中国水产科学研究院南海水产研究所, 广东 广州 510300;中国水产科学研究院南海水产研究所, 广东 广州 510300 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项（2013ZD03）；国家农业财政项目（2009-2013）；广东省科技计划项目（2010B030800008） |
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| 摘 要： | 采用线粒体细胞色素b(cytochrome b,Cyt b)基因片段为遗传标记,分析了南海北部陆架和南沙西南部陆架海域5个黄鳍马面鲀群体的遗传结构,以判定南海北部不同海域之间及南海北部与南沙西南部陆架海域黄鳍马面鲀的种群归属。结果表明,在156个个体的779 bp Cyt b同源序列中共检测到56个变异位点和58种单倍型,5个群体间遗传距离为0.002 95~0.004 15,遗传分化性呈现出高单倍型多样性(0.820 1~0.980 4)和低核苷酸多样性(0.002 62~0.004 69)的特点;分子方差分析和遗传分化指数显示,黄鳍马面鲀的遗传变异来自群体内个体间,群体间无显著遗传分化;单倍型网络结构图和群体系统发育树结构均未出现明显的以地方群体为单位的家系式分支或者聚簇。比较5个不同地理群体间的遗传发育关系并结合种属界定标准判定,南海北部和南沙西南部陆架5个黄鳍马面鲀群体属于同一个种群。
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| 关 键 词： | 黄鳍马面鲀 种群分析 线粒体细胞色素b基因 遗传分化 南海 |
| 收稿时间： | 2013/8/19 0:00:00 |
| 修稿时间： | 2013/11/7 0:00:00 |
Population analysis of Thamnaconus hypargyreus based on the mitochondrial Cyt b sequence |
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| LI Yufang,LI Yongzhen,CHEN Guobao,SUN Dianrong,YU Jie and CUI Ke.Population analysis of Thamnaconus hypargyreus based on the mitochondrial Cyt b sequence[J].Journal of Fisheries of China,2014,38(1):23-32. |
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| Authors: | LI Yufang LI Yongzhen CHEN Guobao SUN Dianrong YU Jie and CUI Ke |
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| Institution: | South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;Regional Bureau of South China Sea Fishery Management, Ministry of Agriculture, Guangzhou 510080, China;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China |
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| Abstract: | Lesser-spotted leatherjacket Thamnaconus hypargyreus is an economically important fish species in the South China Sea.It is necessary to study its genetic structure for the exploitation and management of the fishery resources.Fish specimens(totally 156 individuals)used in this study were collected from 5 sampling locations in the north of the South China Sea and the southwest of Nansha.The results were as follows:the mitochondrial cytochrome b(Cyt b)gene sequences of the fish was amplified by polymerase chain reaction(PCR)technology.Both 56 mutations of nucleotide acids without inserting or deleting one and 58 haplotypes were found among the examined 779 base-pair nucleotide sequences of Cyt b fragment.The ranges and the level of average genetic distance were 0.002 95-0.004 15 for T.hypargyreus between groups.All 5 groups were characterized with high haplotype diversity(0.820 1-0.980 4)and relatively low nucleotide diversity(0.002 62-0.004 69).Analysis of molecular variance(AMOVA)and the fixation indices(Fst)of 5 groups showed that the genetic variance mainly came from individuals within groups,and there was no genetic differentiation between groups.With high gene flow,this group could be developed and managed as an evolutional significant unit.The median-joining(MJ)network and phylogenetic tree proved no phylogeographic differentiation structure in 5 groups.According to the criteria distinguishing different populations,species and genera given by scholar Kartavtsev,the five groups should be merged to the same population,which implied T.hypargyreus in the North and South-West Continental Shelf of the South China Sea belongs to one population.The findings would provide a scientific basis for the study of migration of T.hypargyreus between the two Continental Shelves and management of its stock. |
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| Keywords: | Thamnaconus hypargyreus population analysis mitochondrial cytochrome b genetic differentiation South China Sea |
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