| 利用生物反应器大量生产人参不定根 |
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| 引用本文： | 朴炫春,廉美兰,王守明.利用生物反应器大量生产人参不定根[J].林业科技,2007,32(6):54-56. |
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| 作者姓名： | 朴炫春 廉美兰 王守明 |
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| 作者单位： | 延边大学农学院园艺系,吉林,龙井,133400 |
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| 基金项目： | 国家自然科学基金，教育部科学技术研究重点项目 |
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| 摘 要： | 在5 L气球型生物反应器中,采用完全浸没式培养方式,人参不定根鲜重和干重明显优于间歇浸没式培养;在培养开始时一次性注入含50 g/L蔗糖的培养基,可促进人参不定根鲜重和干重的增加;接种时,将不定根切成1 cm长、接种量为20 g时接种较适宜;增殖培养周期以30天为宜.
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| 关 键 词： | 人参 不定根 生物反应器 |
| 文章编号： | 1001-9499(2007)06-0054-03 |
| 修稿时间： | 2007年5月28日 |
Mass Producing of Adventitious Roots of Ginseng by Using Bioreactor |
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| PIAO Xuanchun.Mass Producing of Adventitious Roots of Ginseng by Using Bioreactor[J].Forestry Science & Technology,2007,32(6):54-56. |
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| Authors: | PIAO Xuanchun |
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| Abstract: | In 5L balloon-type of bioreactor,the adventitious root of ginseng was growth well in immersion culture.Its fresh and dry weight in immersion culture obviously exceed than in ebb-flood culture.The adventitious root growth was promoted by the treatment which the sugar concentration of 50 g/L.Otherwise,It was more suitable that the adventitious roots were cut by 1 cm of size as explants.Besides,inoculation density of 20 g was better.Large numbers of adventitious root growth was found after 30 days of culture period,and it could provide most adventitious root for enhancing ginsenosides at next stage. |
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| Keywords: | Ginseng Adventitious root Bioreactor |
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