| 平胸龟2个地理种群遗传差异的比较 |
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| 引用本文： | 郑光明,马丽莎,朱新平,刘毅辉,陈永乐,罗建仁.平胸龟2个地理种群遗传差异的比较[J].华中农业大学学报,2008,27(4). |
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| 作者姓名： | 郑光明 马丽莎 朱新平 刘毅辉 陈永乐 罗建仁 |
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| 作者单位： | 1. 中国水产科学研究院珠江水产研究所,广州,510380
2. 中国水产科学研究院珠江水产研究所,广州,510380;广东海洋大学水产学院,湛江,524025 |
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| 基金项目： | 广东省自然科学基金项目 |
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| 摘 要： | 利用25个随机引物对平胸龟2个种群共49个个体分别进行PCR扩增,结果显示:平均每个个体扩增出209条DNA带,平均每个引物可产生8条带,扩增谱带分子质量大小在100～2000 bp之间,多态性比例为72.13%.其中12条引物扩增出16个特异于不同种群的DNA片段,可作为鉴别广东或福建种群的分子标记.用RAPD1.04和Genepop3.2软件分析所得谱带,得出广东、福建2个地理种群的平胸龟具有较高的遗传多样性,且广东种群的遗传多样性高于福建种群.2个种群间的平均遗传距离为0.278 6±0.025 9,遗传分化指数Gst为0.285 7.NJTREE聚类分析显示广东和福建2个地区的平胸龟各自聚成2个类群,有较明显的种群分化.
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| 关 键 词： | 平胸龟 地理种群 遗传差异 RAPD分析 |
Genetic Diversity Analysis between Two Populations of Platysternon megacephalum(Gay) by RAPD |
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| ZHENG Guang-ming,MA Li-sha,ZHU Xin-ping,LIU Yi-hui,CHEN Yong-le,LUO Jian-ren.Genetic Diversity Analysis between Two Populations of Platysternon megacephalum(Gay) by RAPD[J].Journal of Huazhong Agricultural University,2008,27(4). |
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| Authors: | ZHENG Guang-ming MA Li-sha ZHU Xin-ping LIU Yi-hui CHEN Yong-le LUO Jian-ren |
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| Abstract: | Genetic diversity between two populations of big-headed turtle was estimated using random-amplified polymorphic DNA(RAPD) assays.49 individuals were amplified using 25 arbitrary primers.Averaged 209 and 8 DNA fragments were produced in each individual and primer respectively.Its polymorphic percentage was 72.13%,the size of DNA fragments from 100 bp to 2 000 bp.16 specific RAPD fragments were sored from 12 primers in two populations,which could be used as RAPD markers for distinguishing the Guangdong and Fu... |
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