| 培养基及培养条件对西洋梨试管苗生根的影响 |
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| 引用本文： | 孙清荣,刘庆忠,赵红军,刘鹏.培养基及培养条件对西洋梨试管苗生根的影响[J].落叶果树,2004,36(1):1-3. |
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| 作者姓名： | 孙清荣 刘庆忠 赵红军 刘鹏 |
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| 作者单位： | 山东省果树研究所,泰安,271000 |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金项目(01DS48) |
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| 摘 要： | 以西洋梨品种丰产(Fertility)和安久(D’Anjou)的试管苗为试材，研究了培养基的组成成分及光暗培养条件对试管苗生根的影响。结果表明：在供试的3种基本培养基中，以1／4MS KNO3最佳；生长素IBA优于NAA；培养基中添加2％的蔗糖优于3％的蔗糖。最佳生根诱导培养基为1／4MS KNO3 0．3mg/LIBA 2％蔗糖，光培养或暗培养3天，生根率达80％以上。
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| 关 键 词： | 培养基 培养条件 西洋梨 试管苗 生根率 组织培养 |
| 文章编号： | 1002-2910(2004)01-0001-03 |
| 修稿时间： | 2003年10月9日 |
Effect of media and culture conditions on root induction of Pyrus cornmunis L.shoots in vitro |
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| SUN Qingrong,LIU Qingzhong,ZHAO Hongjun,LIU Peng.Effect of media and culture conditions on root induction of Pyrus cornmunis L.shoots in vitro[J].Deciduous Fruits,2004,36(1):1-3. |
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| Authors: | SUN Qingrong LIU Qingzhong ZHAO Hongjun LIU Peng |
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| Abstract: | Effect of basic medium components and culture conditions (dark or light) on in vitro shoot rooting of pear (Pyrus communis L.) were studied.Results showed that the 1/4MS KNO_3 (1/4MS plus major inorganic element KNO_3) was the optimal for rooting of cultivars Fertility and D'Anjou in the tested 3 basic media, 1/4MS KNO_3,1/2MS and 1/4QL.The medium amended with IBA was better than that with NAA. As to the level of sugar added, the 2% was superior to the 3%. The 1/4MS KNO_3 + 0.3mg/L IBA + 2% sugar was the best medium for rooting, culture on which for 3d gave more than 80% of rooting rate. |
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| Keywords: | pear in vitro shoots rooting |
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