Use of molecular markers aids in the development of diverse inbred backcross lines in Beit Alpha cucumber (<Emphasis Type="Italic">Cucumis sativus</Emphasis> L.) |
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| Authors: | Isabelle Y Delannay Jack E Staub |
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| Institution: | (1) Vegetable Crops Unit, U.S. Department of Agriculture, Agricultural Research Service, Department of Horticulture, University of Wisconsin, 1575 Linden Dr, Madison, WI 53706, USA;(2) Present address: U. S. Department of Agriculture, Agricultural Research Service, Forage and Range Research Laboratory, Utah State University, Logan, UT 84322-6300, USA |
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| Abstract: | Beit Alpha cucumber (Cucumis sativus L.) is a Mediterranean fresh-market type with a relatively narrow genetic base. To broaden its base for plant improvement,
42 diverse accessions were compared employing a previously defined standard marker array to choose wide-based parental lines
for use in backcross introgression. Inbred backcross lines (IBL) were developed by crossing Beit Alpha line ‘04HD5’ (De Ruiter
Seeds, The Netherlands; recurrent parent) and PI 285606 (Poland; donor parent), and then selecting the most genetically diverse
BC1 and BC2 progeny based on molecular marker profiles, followed by three generations of single-seed descent to produce 117 IBL. Molecular
genotyping of IBL was then performed, and IBL were evaluated for days to anthesis, sex expression, pistillate flowers per
node, lateral branch number, fruits per plant, fruit length, and fruit weight in the US, The Netherlands, Israel, and Turkey.
Multivariate analyses and genetic distance comparisons indicate that IBL possessed considerable inter-line morphological and
genotypic diversity. These diverse IBL will be useful in genetic studies and to evaluate Beit Alpha cross-progeny derived
from IBL × elite germplasm created to broadened genetic base of this market type. |
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