| 利用PCR技术初步鉴定小麦-加州野大麦异染色体系 |
| |
| 引用本文： | 王秀娥,赵彦,张清平,王苏玲,周波,陈佩度,刘大钧.利用PCR技术初步鉴定小麦-加州野大麦异染色体系[J].南京农业大学学报,2004,27(4):1-5. |
| |
| 作者姓名： | 王秀娥 赵彦 张清平 王苏玲 周波 陈佩度 刘大钧 |
| |
| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室,细胞遗传研究所,江苏,南京,210095 |
| |
| 基金项目： | 国家高技术研究发展863计划(2001AA211051,2003AA211031) |
| |
| 摘 要： | 为快速鉴定普通小麦与普通小麦—加州野大麦双二倍体杂交、回交后代植株的染色体组成，研究小麦背景中添加的外源染色体与小麦染色体之间的部分同源关系，选用已被定位在小麦7个部分同源群21条染色体上的38个SSR引物对杂种回交后代植株进行PCR扩增。结果表明，其中27个小麦SSR引物在普通小麦与小麦—加州野大麦双二倍体间有多态性扩增，涉及4个部分同源群的11对引物，可在不同杂种回交植株中扩增出与双二倍体相同的多态带纹；根据PCR扩增和细胞遗传学分析的结果，在18个回交后代中初步鉴定出7个可能的异附加系，其中2个二体异附加系、1个端二体异附加系、2个单体异附加系和2个双单体异附加系。所选育的异附加系分别涉及第1、2、4和7部分同源群。
|
| 关 键 词： | 小麦 加州野大麦 异染色体系 简单重复序列 |
| 文章编号： | 1000-2030(2004)04-0001-05 |
| 修稿时间： | 2003年12月30 |
Preliminary identification of Triticum aestivum L.-Hordeum californicum alien chromosome lines by PCR technique |
| |
| WANG Xiu-e,ZHAO Yan,ZHANG Qing-ping,WANG Su-ling,ZHOU Bo,CHEN Pei-du,LIU Da-jun.Preliminary identification of Triticum aestivum L.-Hordeum californicum alien chromosome lines by PCR technique[J].Journal of Nanjing Agricultural University,2004,27(4):1-5. |
| |
| Authors: | WANG Xiu-e ZHAO Yan ZHANG Qing-ping WANG Su-ling ZHOU Bo CHEN Pei-du LIU Da-jun |
| |
| Abstract: | For rapid identification of alien chromosome lines among BC 2F 2 plants as well as characterization of the homoeologous relationships between the added H.californicum chromosomes and wheat chromosomes,PCR was conducted on 18 plants with chromosome number 2n=43 or 2n=44 using 38 wheat SSR primers having mapped on 21 wheat chromosomes.The results indicated that 27 primers could amplify polymorphic bands between Chinese Spring and the amphiploid.The identified polymorphic primers were further used to identify the selected 18 plants,11 primers could amplify the same specific bands in different BC 2F 2 plants as in the amphiploid.7 alien addition lines were identified including 2 disomic additions,1 ditelosomic addition,2 monosomic additions and 2 double monosomic additions.The identified addition lines involved H.californicum chromosomes of homoeologous group 1,2,4 and 7. |
| |
| Keywords: | Triticum aestivum Hordeum californicum alien chromosome lines simple sequence repeat(SSR) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
