| DNA分子原位杂交在植物分子细胞遗传学研究中的应用 |
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| 引用本文： | 别同德,张伯桥,高德荣,程顺和,马谈斌.DNA分子原位杂交在植物分子细胞遗传学研究中的应用[J].中国农学通报,2008,24(11):85-92. |
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| 作者姓名： | 别同德 张伯桥 高德荣 程顺和 马谈斌 |
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| 作者单位： | 扬州农科院国家小麦改良分中心,江苏扬州,225007 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，国家科技支撑计划 |
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| 摘 要： | DNA分子原位杂交（in situ hybridiation）是植物分子细胞遗传学研究的重要工具。本文简要回顾了DNA分子原位杂交的起源和发展，详细综述了基因组原位杂交（genomic in situ hybridization, GISH）在植物细胞遗传学研究中的应用以及荧光原位杂交（Fluorescence in situ hybridization, FISH）在物理作图和染色体识别中的应用。文中还介绍了Fiber-FISH、BAC-FISH以及Immuno-FISH等新兴技术。最后对FISH技术进行了展望。
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| 关 键 词： | FISH GISH ISH 分子细胞遗传学 植物 |
| 收稿时间： | 2008-08-28 |
| 修稿时间： | 2008-09-09 |
Application of in situ Hybridization in Plant Molecular Cytogenetics Research |
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| BIE Tong-De,derong gao.Application of in situ Hybridization in Plant Molecular Cytogenetics Research[J].Chinese Agricultural Science Bulletin,2008,24(11):85-92. |
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| Authors: | BIE Tong-De derong gao |
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| Abstract: | In situ hybridization is one of the most important techniques in plant molecular cytogenetics research.In this paper, we briefly introduced the origin and development of in situ hybridization, firstly.Then, application of genomic in situ hybridization(GISH) in plant cytogenetics research as well as fluorescence in situ hybridization(FISH) in plant physical mapping and chromosome identification were detailed reviewed.Some new techniques such as fiber-FISH, BAC-FISH, and immuno-FISH were also introduced in this paper.And a prospect about FISH was given out. |
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| Keywords: | FISH GISH ISH |
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