Pregnancy rate after fixed‐time transfer of cryopreserved embryos collected by non‐surgical route in Lacaune sheep |
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| Authors: | Lucas Machado Figueira Nadja Gomes Alves Ribrio Ivan Tavares Pereira Batista Viviane Lopes Brair Renato Ribeiro Lima Maria Emilia Franco Oliveira Jeferson Ferreira Fonseca Joanna Maria Gonalves Souza‐Fabjan |
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| Institution: | Lucas Machado Figueira,Nadja Gomes Alves,Ribrio Ivan Tavares Pereira Batista,Viviane Lopes Brair,Renato Ribeiro Lima,Maria Emilia Franco Oliveira,Jeferson Ferreira Fonseca,Joanna Maria Gonçalves Souza‐Fabjan |
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| Abstract: | This study investigated the feasibility of applying fixed‐time (cryopreserved) embryo transfer in ewes. Embryos (n = 106) were non‐surgically recovered from superovulated donors (n = 39) on day 6–7 after oestrus. Straws containing one or two embryos (morulae and/or blastocysts) subjected to either slow freezing (SF, n = 62) or vitrification (VT, n = 44) were randomly used within fixed‐time embryo transfer on Day 8.5. Recipient ewes were nulliparous (n = 58) bearing corpora lutea after synchronous oestrous induction protocol. The pregnancy rate was higher (p = .03) in SF (39.4%) than VT (16.9%) and survival rate tended (p = .08) to be higher in SF than in VT (25.8% vs. 15.9%). Lambing rates were similar (p = .13) between SF (20.9%) and VT (15.9%). Embryos recovered by non‐surgical route after cervical dilation treatment and later cryopreserved by either slow freezing or vitrification produced reasonable pregnancy rates after FTET. |
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| Keywords: | embryo cryopreservation multiple ovulation and embryo transfer ovine |
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