| 应用荧光定量PCR筛选绵羊肺炎支原体最适生长培养基 |
| |
| 引用本文： | 张双翔,程振涛,周碧君,文明,王开功,李泽民,覃岚,崔亚兰,王慧.应用荧光定量PCR筛选绵羊肺炎支原体最适生长培养基[J].畜牧与兽医,2013,45(1):9-14. |
| |
| 作者姓名： | 张双翔 程振涛 周碧君 文明 王开功 李泽民 覃岚 崔亚兰 王慧 |
| |
| 作者单位： | 1. 贵州大学动物科学学院,贵州贵阳,550025
2. 贵州大学动物科学学院,贵州贵阳550025;贵州省动物疫病研究室,贵州贵阳550025 |
| |
| 基金项目： | 贵州省农业科技攻关项目(黔科合NY字[2011-3105]号);贵州省自然科学技术基金(黔科合J字[2011]2332号);贵州大学引进人才科研项目(贵大人基合字(2010)35号);黔西南州种草养羊产业发展省州科技合作专项项目(黔西南科合[2012]5号);贵州大学2012年研究生创新基金项目(校基金农科2012034) |
| |
| 摘 要： | 为筛选绵羊肺炎支原体(Mo)最适生长培养基,针对Mo 16S rRNA基因设计特异性引物FQs/FQa,建立可定量检测Mo 16S rRNA基因FQ-PCR方法,通过检测不同培养时间各Mo培养液中Mo Y98株核酸含量,比较Mo于不同培养基中培养特性,以筛选Mo最适生长培养基。结果,以Broth-ame与TSB1培养Mo 7,10和14 d时Mo核酸检测值较高,较Frey-ame、PPLO-ame更适合Mo生长。本研究为后续Mo生物学特性分析奠定了基础。
|
| 关 键 词： | 荧光定量PCR 绵羊肺炎支原体 生长培养基 |
Application of fluorescent quantitative PCR in selecting the best growth medium of Mycoplasma ovipneumoniae |
| |
| ZHANG Shuang-xiang,CHENG Zhen-tao,ZHOU Bi-jun,WEN Min,WANG Kai-gong,LI Ze-min,QIN Lan,CUI Ya-lan,WANG Hui.Application of fluorescent quantitative PCR in selecting the best growth medium of Mycoplasma ovipneumoniae[J].Animal Husbandry & Veterinary Medicine,2013,45(1):9-14. |
| |
| Authors: | ZHANG Shuang-xiang CHENG Zhen-tao ZHOU Bi-jun WEN Min WANG Kai-gong LI Ze-min QIN Lan CUI Ya-lan WANG Hui |
| |
| Institution: | 1(1.College of Animal Science,Guizhou University,Guiyang 550025,China; 2.Laboratory for Animal Disease of Guizhou,Guiyang 550025,China) |
| |
| Abstract: | To select the best growth medium of Mycoplasma ovipneumoniae(Mo),the experiment was conducted to compare the culture characteristics of Mo in different media by detecting nucleic acid contents of Mo Y98 strain.A pair of primers FQs/Fqa was designed to amplify 16S rRNA gene of Mo by fluorescent quantitative PCR.The results showed that Mo grew better in Broth-ame and TSB1 rather than in Frey-ame and PPLO-ame,as the nucleic acid detecting values of Mo at 7d,10d and 14d were higher.This study provides a foundation for understanding the biological characterization of Mo. |
| |
| Keywords: | fluorescent quantitative PCR Mycoplasma ovipneumoniae growth medium |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
