| 井冈霉素高产菌株原生质体诱变筛选研究 |
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| 引用本文： | 覃敬羽,李世杰,刘华梅.井冈霉素高产菌株原生质体诱变筛选研究[J].现代农业科技,2010(22):38-39. |
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| 作者姓名： | 覃敬羽 李世杰 刘华梅 |
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| 作者单位： | 1. 湖北工业大学生物工程学院,湖北武汉,430068
2. 武汉科诺生物科技有限公司 |
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| 摘 要： | 以吸水链霉菌井冈变种k-121-5为出发菌株制备原生质体,并对其进行紫外诱变,通过筛选得到5株较为高产的菌株,经连续传代、摇瓶发酵,井冈霉素A产率比出发菌株提高14%～26%。
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| 关 键 词： | 井冈霉素 吸水链霉菌井冈变种 原生质体 诱变 筛选 |
Research on Screening of Jinggangmycin strain with High-yield by Protoplast Mutation |
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| Authors: | QIN jing-yu LI shi-jie LIU Hua-mei |
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| Institution: | QIN jing-yu 1 LI shi-jie 1 LIU Hua-mei 2(1 College of Biological Engineering,Hubei University of Technology,Wuhan Hubei 430068;2 Wuhan Kernel Bio-tech Co.Ltd) |
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| Abstract: | treptomyces hygroscopicus var.jinggangensis Yen K-121-5 was accomplished protoplasts preparation.The protoplasts were treated with ultraviolet irradiation,screened on culture plate and obtained 5 high high-yield strains.Jinggangmycin A yields of the mutation strains was 14%～ 26% higher than the original strain by shaking flask fermentation after series transfer culturing. |
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| Keywords: | Jinggangmycin Streptomyces hygroscopicus var jinggangensis Yen protoplast mutation screening |
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