| 蔗糖代谢相关酶在温州蜜柑果实糖积累中的作用 |
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| 引用本文： | 赵智中,张上隆,徐昌杰,陈昆松,刘拴桃.蔗糖代谢相关酶在温州蜜柑果实糖积累中的作用[J].园艺学报,2001,28(2):112-115. |
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| 作者姓名： | 赵智中 张上隆 徐昌杰 陈昆松 刘拴桃 |
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| 作者单位： | ( 浙江大学园艺系, 杭州310029 ; 浙江大学生命科学学院, 杭州310029) |
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| 基金项目： | 国家自然科学基金重点资助项目(39730340) |
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| 摘 要： | 在不同发育时期测定了宫川温州蜜柑果实中蔗糖、葡萄糖和果糖含量以及蔗糖代谢相关酶———酸性转化酶(AI) 、中性转化酶(NI) 、蔗糖合成酶(SS) 和蔗糖磷酸合成酶(SPS) 的活性, 并对果实中糖积累与酶活性的关系进行了分析。结果表明: 蔗糖代谢相关酶的综合作用(蔗糖代谢相关酶的净活性) 是影响果实糖积累的重要因子之一。膨大期末至着色期初是果实中糖积累和蔗糖代谢相关酶的净活性变化的转折时期。着色期前果实中蔗糖代谢相关酶的净活性为负值, 蔗糖缓慢积累; 进入着色期蔗糖代谢相关酶的净活性转为正值,蔗糖积累迅速。完熟期果皮组织中蔗糖代谢相关酶的净活性为负值, 己糖积累明显高于可食组织。
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| 关 键 词： | 柑桔类 温州蜜柑 糖积累 转化酶 蔗糖合成酶 蔗糖磷酸合成酶 |
| 文章编号： | 0513-353X(2001)02-0112-07 |
| 修稿时间： | 2000年10月19 |
Roles of
Sucrose-Metabolizing Enzymes in Accumulation of Sugars in Satsuma Mand arin Fruit |
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| Zhao Zhizhong ,Zhang Shanglong ,Xu Changjie ,Cheng Kunsong ,and Liu Shuantao.Roles of
Sucrose-Metabolizing Enzymes in Accumulation of Sugars in Satsuma Mand arin Fruit[J].Acta Horticulturae Sinica,2001,28(2):112-115. |
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| Authors: | Zhao Zhizhong Zhang Shanglong Xu Changjie Cheng Kunsong and Liu Shuantao |
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| Institution: | 1. Department of Anatomy, Guangzhou Medical College, Guangzhou, 510182, China;
2. Molecular Cell Biology, Division of Molecular and Genetic Medicine, Sheffield University, UK |
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| Abstract: | The content of sucrose,glucose,fructose and the activities of sucrose metabolizing enzymes(acid invertase,neutral invertase,sucrose synthase and sucrose phosphate synthase)in the fruits of Citrus unshiu Marc.cv.Miyagawa wase were measured.It was observed that compositive action of sucrose metabolizing enzymes(net activity of sucrose metabolizing enzymes)was one of the important factors affecting sugar accumulation in fruit.The transition of sugar accumulation and net activity of sucrose metabolizing enzymes was the period from the end of fruit enlargement to the onset of fruit coloring in fruits.Before the stage of fruit coloring,the net activity of sucrose metabolizing enzymes was below zero,sucrose accumulated slowly in fruit.During stage of fruit coloring,the net activity of sucrose metabolizing enzymes was positive,and sucrose accumulated rapidly in fruit.During the stage of fruit full ripe the net activity of sucrose metabolizing enzymes was negative in peel tissue,and accumulation of hexoses was higher in comparison with edible tissue. |
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| Keywords: | Citrus Citrus unshiu Marc cv Miyagawa wase Accumulation of sugars Invertase Sucrose synthase Sucrose phosphate synthase |
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