| 基于SSR标记的梨资源遗传多样性分析 |
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| 引用本文： | 张妤艳,吴俊,张绍铃.基于SSR标记的梨资源遗传多样性分析[J].农业生物技术学报,2008,16(6). |
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| 作者姓名： | 张妤艳 吴俊 张绍铃 |
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| 作者单位： | 1. 南京农业大学园艺学院,南京,210095;江苏省农业科学院园艺研究所,南京,210014
2. 南京农业大学园艺学院,南京,210095 |
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| 基金项目： | 国家自然科学基金，教育部科学技术研究项目 |
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| 摘 要： | 本研究利用SSR标记技术对56份梨资源进行了遗传多样性分析。利用筛选出的6对SSR引物共扩增40条谱带，其中多态性位点38个，多态性位点比例为95%，每对引物产生有效等位基因6.3个。各位点期望杂合度H值在0.0354～0.491，平均为0.1964；有效等位基因Ne值在1.0367～1.9648，平均值为1.2958；香农指数I值平均值为0.3256，说明了供试梨材料的遗传多样性较低。利用SSR标记可将44个栽培品种区分开，但无法区分芽变和原种。根据SSR标记揭示的多态性，采用NTSYS-pc软件，以UPGMA法进行聚类分析，结果显示所检测的56份梨材料在相似系数0.71处可分为4组，其中中国的白梨、秋子梨和砂梨相互交错在一起，没有独自各自成组。
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| 关 键 词： | 梨 SSR 遗传多样性 亲缘关系 |
| 收稿时间： | 2008-2-1 |
| 修稿时间： | 2008-4-21 |
Genetic Diversity of Germplasm Resources of Pyrus Based on SSR Marker |
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| ZHANG Yu-yan,WU Jun,ZHANG Shao-ling.Genetic Diversity of Germplasm Resources of Pyrus Based on SSR Marker[J].Journal of Agricultural Biotechnology,2008,16(6). |
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| Authors: | ZHANG Yu-yan WU Jun ZHANG Shao-ling |
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| Abstract: | Levels of genetic diversity within and among 56 germplasm of Pyrus were analyzed using SSR markers. 40 bands were amplified by six informative and reliable prmiers screened, of which 38 (95% ) were polymorphic, with an average of 6.3 alleles per locus. Nei’s gene diversity index ranged from 0.0354 to 0.491 with an average of 0.1964 , effective number of alleles range from1.0367 to 1.9648 with an average of 1.2958. Genetic diversity difference among tested pear cultivars was relatively low based on Shannon index (0.3256). 44 pear cultivars could be distinguished by SSR markers except the mutant cultivars. UPGMA cluster analysis was performed with soft NTYSIS-pc2.01, the clades formed including four populations at the dice coefficient of 0.71. Pyrus bretschneideri Rehd., P. ussuriensis Maxim. and P. pyrifolia Nakai. originating in China interveined each other and could not form group independently . |
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| Keywords: | Pyrus SSR genomic polymorphism genetic relationship |
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