| 不孕奶牛血液生理生化指标分析 |
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| 引用本文： | 张玥琨,黄启震,陈超磊,王相国,盛熙晖,齐晓龙,郭勇,王天坤,倪和民,常迪.不孕奶牛血液生理生化指标分析[J].中国农学通报,2018,34(6):114-117. |
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| 作者姓名： | 张玥琨 黄启震 陈超磊 王相国 盛熙晖 齐晓龙 郭勇 王天坤 倪和民 常迪 |
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| 作者单位： | 北京农学院,北京农学院,北京农学院,北京农学院,北京农学院,北京农学院,北京农学院,昌平区动物疫病预防控制中心,北京农学院,北京农学院 |
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| 基金项目： | 北京市奶牛产业创新团队(BAIC05-2017)；国家重点研发计划“畜禽重大疫病防控与高效安全养殖综合技术研发”重点专项“畜禽群发普通 病防控技术研究”(2017YFD0502200)；三项基金-青年教师科研基金“奶牛子宫内膜炎引起不孕的机制研究”(5056016013/003)。 |
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| 摘 要： | 为了探究奶牛不孕症的发病特点，有效防治不孕症的发生。本试验对患有久配不孕、子宫增生、子宫内膜炎、卵巢囊肿的奶牛进行血液生理生化指标测定。采用全自动血细胞分析仪和Vet Test生化分析仪对患病奶牛与健康奶牛的全血进行血液常规指标和生化指标检测。发现子宫内膜炎引起白细胞数量显著增多（P<0.05）；而红细胞数、血红蛋白含量、淋巴细胞百分数、单核细胞百分数和中性粒细胞百分数在四种病例中均无显著变化；卵巢囊肿可导致血液总蛋白量（TP）显著增加(P<0.05)；丙氨酸转氨酶（ALT）、碱性磷酸酶（ALKP）和血糖（GLU）在各种病例中均无显著变化（P>0.05）；久配不孕、子宫内膜炎和卵巢囊肿均能显著降低血液中钙离子（Ca）浓度（P<0.05）；并且，久配不孕、子宫内膜炎和卵巢囊肿均能显著增高血液中磷离子（PHOS）浓度（P<0.05），打破钙磷平衡；同时，在所有分组中发现，血糖浓度均低于正常标准值。由此可见，血液中钙磷平衡可以作为辅助指标预测奶牛不孕症的发生。在早期疾病诊断过程中，保持血液中钙磷比例平衡是防治奶牛不孕症发生的重要手段之一。
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| 关 键 词： | 不孕症 子宫增生 子宫内膜炎 卵巢囊肿 生理生化指标 |
| 收稿时间： | 2017/10/14 0:00:00 |
| 修稿时间： | 2017/12/14 0:00:00 |
Analysis of Blood Physiological and Biochemical Indexes in Infertile Cows |
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| Abstract: | To investigate the clinical characters of cows’infertility and prevent and cure infertility effectively, the blood physiological and biochemical indexes of cows with infertility, uterus hyperplasia, endometritis or ovarian cyst were measured. Conventional blood indexes and biochemical indexes of diseased cows and healthy cows were tested by automatic blood cell analyzer and Vet Test biochemical analyzer. Results showed that endometritis caused a significant increase of white blood cells (P <0.05); but red blood cell count, hemoglobin content and lymphocytes percentage, monocytes percentage and neutrophils percentage had no significant change in the four cases. Ovarian cyst caused a significant increase of total protein (TP) in blood (P <0.05). There was no significant change in regard to alanine aminotransferase (ALT), alkaline phosphatase (ALKP) and blood glucose (GLU) in all cases (P >0.05); infertility, endometritis, and ovarian cyst could significantly reduce the concentration of calcium(Ca) in blood (P <0.05); besides, infertility, endometritis, and ovarian cyst could significantly increase the concentration of phosphorus ions (PHOS) in blood and break the balance of calcium
and phosphorus (P <0.05); meanwhile, it was found in all groups that blood glucose concentration was lower than normal standard value. Thus, it indicated that the balance of calcium and phosphorus in blood can predict the infertility of cows as an auxiliary index. Additionally, keeping the balance of calcium and phosphorus in blood is one of the effective means to prevent and cure the infertility of cows in the early diagnosis of disease. |
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| Keywords: | Infertility uterine hyperplasia endometritis ovarian cyst physiological and biochemical indexes |
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