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5个猪种DECR1基因外显子2单链构象多态性的群体遗传学分析
引用本文:李根银,王效京,曹宁贤,刘宏,张维军,曹果清,李步高.5个猪种DECR1基因外显子2单链构象多态性的群体遗传学分析[J].中国畜牧兽医,2010,37(7):128-131.
作者姓名:李根银  王效京  曹宁贤  刘宏  张维军  曹果清  李步高
作者单位:(1.山西省畜禽繁育工作站,太原 030000;2.大同市种猪场,大同 037000;3.山西省长治市畜牧局,长治 046000;4.山西农业大学动物科技学院,太谷 030801)
基金项目:山西省科技攻关项目,国家农业科技成果转化项目 
摘    要:试验利用PCR-SSCP技术检测了山西马身猪、山西白猪、长白猪、大白猪和杜洛克猪5个品种共490头猪的DE-CR1基因外显子2的多态性。结果显示,DECR1基因外显子2位点存在多态性,表现出AA、BB和AB 3种基因型。群体遗传学分析结果表明,5个猪种都是等位基因B为优势基因;Hardy-Weinberg平衡检验结果显示,山西白猪和杜洛克猪处于平衡状态,山西马身猪、长白猪和大白猪处于非平衡状态;各品种猪的多态信息含量均为中度多态。

关 键 词:DECR1基因  PCR-SSCP  多态性    

Population Genetic Analysis of Polymorphisms on Exon 2 of DECR1 Gene in 5 Pig Breeds
LI Gen-yin,WANG Xiao-jing,CAO Ning-xian,LIU Hong,ZHANG Wei-jun,CAO Guo-qing,LI Bu-gao.Population Genetic Analysis of Polymorphisms on Exon 2 of DECR1 Gene in 5 Pig Breeds[J].China Animal Husbandry & Veterinary Medicine,2010,37(7):128-131.
Authors:LI Gen-yin  WANG Xiao-jing  CAO Ning-xian  LIU Hong  ZHANG Wei-jun  CAO Guo-qing  LI Bu-gao
Institution:(1.Shanxi Livestock and Poultry Breeding Station,Taiyuan 030000,China;2. Datong Pig Breeding Farm in Shanxi Province,Datong 037000,China;3. Bureau of Livestock of Changzhi City in Shanxi Province,Changzhi 046000,China;4. College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)
Abstract:The polymorphisms of the 2,4-dienoyl CoA redutase 1 (DECR1) gene in exon 2 site was detected using PCR-SSCP in 490 pigs of 5 pig breeds including Shanxi Mashen, Shanxi White, Landrace, Yorkshire and Duroc. The results showed that there was a polymorphism in the exon 2 site and three genotypes (AA, BB and AB) were found. Analysis of population genetics suggested that the frequency of B allele was dominant among the studied breeds. The results of Hardy-Weinberg equilibrium test indicated that Shanxi White and Duroc reached the equilibrium. All of the breeds had intermediate polymorphism information contents.
Keywords:PCR-SSCP
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