| 1株粘细菌CMC0606的分离纯化及其抗菌活性研究 |
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| 引用本文： | 殷建华,代富英,李晋川.1株粘细菌CMC0606的分离纯化及其抗菌活性研究[J].安徽农业科学,2010,38(9):4445-4446,4449. |
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| 作者姓名： | 殷建华 代富英 李晋川 |
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| 作者单位： | 成都医学院病原生物学教研室,四川成都,610083;成都医学院病原生物学教研室,四川成都,610083;成都医学院病原生物学教研室,四川成都,610083 |
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| 基金项目： | 四川省科技厅应用基础研究项目(05JY029-118) |
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| 摘 要： | 目的]对成都西岭雪山土壤中粘细菌进行分离鉴定,了解其发酵产物粗提物的抗菌活性。方法]以滤纸为唯一碳源,得到1株粘细菌CMC0606;通过观察其形态及16SrDNA克隆与测序进行菌种鉴定;向其发酵液中加入大孔吸附树脂XAD-16,收获发酵产物粗提物。结果]菌株CMC0606被鉴定为珊瑚状珊瑚球菌Corallococcus coralloides CMC0606;其发酵产物粗提物对枯草芽孢杆菌、金黄色葡萄球菌及辣椒疫霉具有抑制作用。结论]菌株CMC0606具有抗细菌和抗真菌的活性,尤其对植物致病菌辣椒疫霉抑制作用显著,具有潜在的研究和开发价值。
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| 关 键 词： | 粘细菌 珊瑚球菌 粗提物 抗菌活性 |
Isolation and Purification of CMC0606 and Study on Its Antibiotic Activity |
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| YIN Jian-hua et al.Isolation and Purification of CMC0606 and Study on Its Antibiotic Activity[J].Journal of Anhui Agricultural Sciences,2010,38(9):4445-4446,4449. |
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| Authors: | YIN Jian-hua |
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| Institution: | Department of Pathogenic Organism;Chengdu Medical College;Chengdu;Sichuan 610083 |
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| Abstract: | Objective] Myxobacteria in Xiling Snow Mountain of Chengdu was isolated and purified,which aimed to learn the antibiotic activity of crude extracts. Method] With filter paper as the sole carbon source,a Myxobacteria CMC0606 was obtained; through observing its morphology,16S rDNA cloning and sequencing,the bacteria was identified; adding XAD-16 into fermentation broth,crude extracts of fermentation products were obtained. Result] CMC0606 was identified as Corallococcus coralloides; the crude extracts of i... |
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| Keywords: | Myxobacteria Corallococcus coralloides Crude extract Antibiotic activity |
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