| 豆科牧草沙打旺抗乙硫氨酸变异系筛选 |
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| 引用本文： | 罗建平,贾敬芬,顾月华.豆科牧草沙打旺抗乙硫氨酸变异系筛选[J].作物学报,2000,26(6):789-794. |
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| 作者姓名： | 罗建平 贾敬芬 顾月华 |
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| 作者单位： | 合肥工业大学生物与食品工程系， 安徽合肥， 230069 |
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| 基金项目： | 国家自然科学基金和博士后科学基金资助 |
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| 摘 要： | 以NaN3诱变处理的沙打旺胚性愈伤组织为材料，用甲硫氨酸类似物—乙硫 氨酸为选择剂，筛选到1株抗性稳定且能再生植株的抗0.6 mmol L－1乙硫氨酸 变异系。该变异系细胞对乙硫氨酸的抗性是野生型细胞的8倍。当把变异系愈伤组织传入 体细胞 胚胎诱导培养基上，平均体细胞胚胎发生频率为13.9%，平均每g鲜重愈伤组织可产生21 个体
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| 关 键 词： | 沙打旺 抗乙硫氨酸变异系 体细胞胚胎发生 植株再生 |
| 收稿时间： | 1999-03-26 |
| 修稿时间： | 1999-03-26 |
Selection of Ethionine-resistant Variant in the Forage Legume Astragalus adsurgens Pall |
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| LUO Jian Ping,JIA Jing Fen,GU Yue Hua.Selection of Ethionine-resistant Variant in the Forage Legume Astragalus adsurgens Pall[J].Acta Agronomica Sinica,2000,26(6):789-794. |
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| Authors: | LUO Jian Ping JIA Jing Fen GU Yue Hua |
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| Institution: | Department of Biology and Food Fechnology, Hefei University of Technology, Hefei 230069 |
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| Abstract: | One stable variant of Astragalus adsurgens resistant to 0.6 mmol L -1 ethionine was isolated from embryogenic calli mutagenized with 0.1 mol L - 1 NaN 3 for 3 hours. The resistance of variant to ethionine was 7 times higher than that of the wild type. The variant calli were capable of regenerating plan tlets through somatic embryogenesis in the differentiation medium without ethion ine, and the mean frequency of calli producing somatic embryos and the number of somatic embryos per gram fresh weight callus were 13.9% and 21.4, only 22.9% an d 11.1% of the wild type, respectively. The variant callus and its regenerants an alyzed for free amino acid content were found to contain 2.5 times and 4 times a s much methionine as the wild type, respectively suggesting that its resistance to ethionine was due to the increase in free methionine level. Stem segments from the variant regenerants showed similar callus formation on both normal and 0.6 mmol/L ethionine containing medium while stem segments of the wild type re generants only formed callus on ethionine free medium. This result indicated cl early that ethionine resistance was expressed during callus induction and growth of the variant. |
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| Keywords: | Astragalus adsurgens Pall Ethionine re sistant variant Somatic embryogenesis Plant regeneration |
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