| 甘蔗茎RNA提取方法研究（摘要）（英文） |
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| 引用本文： | 陈萍,罗挺,蔡文伟.甘蔗茎RNA提取方法研究（摘要）（英文）[J].农业科学与技术,2010,11(3):43-46. |
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| 作者姓名： | 陈萍 罗挺 蔡文伟 |
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| 作者单位： | [1]海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南儋州571737 [2]海南大学园艺园林学院,海南儋州571737 [3]中国热带农业科学院生物科学与技术研究所,海南海口571107 |
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| 基金项目： | 海南大学校基金项目（hd09xm62）;海南省教育厅高等学校科学研究资助性项目（Hjkj2010-17）;（原）华南热带农业大学科技基金（RndD710）. |
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| 摘 要： | 目的]找出提取甘蔗茎RNA的适宜方法。方法]采用SDS法、试剂盒法、GHCL法3种方法提取甘蔗茎基因组RNA,并对提取的RNA进行质量比较。结果]试剂盒法提取的RNA产率高,电泳条带清晰,RNA完整性好,无明显降解,OD260/OD280比值较好。试验操作要点：在RNA的提取过程中必须使操作温度保持在4℃左右（操作时温度可以通过垫加冰块来实现）,从而充分抑制RNase的活性;植物组织称取要适量,将植物组织在液氮中充分研磨后,应在液氮还未充分挥发前将研磨物加入Eppendorf管中,样品要充分研磨,通过加大提取液中变性剂的量防止在此过程中内源RNase将植物组织中RNA降解;在用酚/氯仿/异戊醇抽提及氯仿/异戊醇抽提过程中,吸取上清液时,应尽量小心,不要吸取中间层从而防止酚类、蛋白质类的干扰。此外,提取的RNA还应及时保存于-70℃低温下,避免频繁冻融影响其质量以及RNA的降解。结论]试剂盒法是有效提取甘蔗茎RNA的方法。该研究为甘蔗的分子生物学研究奠定了基础。
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| 关 键 词： | 甘蔗 茎 RNA 提取方法 |
Comparison of RNA Extraction Methods of Sugarcane Stem |
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| CHEN Ping,LUO Ting,CAI Wen-wei.Comparison of RNA Extraction Methods of Sugarcane Stem[J].Agricultural Science ＆ Technology,2010,11(3):43-46. |
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| Authors: | CHEN Ping LUO Ting CAI Wen-wei |
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| Institution: | 1.Key Laboratory of Protection,Development and Utilization of Tropical Crop Germplasm Resources of Hainan University,Ministry of Education,Danzhou 571737;2.College of Horticulture and Landscape Architecture,Hainan University,Danzhou 571737;3.Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101 |
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| Abstract: | Objective] The study was to explore the most effective and feasible method for sugarcane stalks RNA extraction.Method] SDS extraction method,kit extraction method and GHCL extraction method were used to extract the sugarcane RNA in stalks,and the quality of the extracted RNA was compared.Result] RNA extracted by kit extraction method had a high-yield,the bands were clear and RNA had a good integrity,there was no significant degradation of RNA,and the OD260 nm/OD280 nm value was closed to 2.0.Conclusion] Kit extraction method was the effectively method to extract sugarcane RNA,and this study had provided a theoretical basis for the molecular biology study of sugarcane. |
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| Keywords: | Sugarcane Stalk RNA extraction method |
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