| 辛基酚对鲤的雌激素效应 |
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| 引用本文： | 宫向红,徐英江,任利华,张世娟,张秀珍,张利民.辛基酚对鲤的雌激素效应[J].水产学报,2010,34(3):410-414. |
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| 作者姓名： | 宫向红 徐英江 任利华 张世娟 张秀珍 张利民 |
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| 作者单位： | 山东省海洋水产研究所,山东,烟台,264006 |
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| 基金项目： | 山东省科技攻关项目(2008GG10005005); 山东省水生动物营养与饲料泰山学者岗位资助 |
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| 摘 要： | 研究了辛基酚对鲤的雌激素效应。经10、50、100、300和500μg/L辛基酚暴露32d后,鲤存活率和肥满度与对照组无差异;性腺指数变化明显,雌鱼性腺指数随暴露剂量的增大而增大,雄鱼性腺指数随暴露剂量的增大而减小;50μg/L及以上暴露组与对照组差异显著(P<0.05)。辛基酚能诱导鲤雄鱼产生卵黄蛋白原,暴露剂量为10μg/L组有部分雄鱼肝脏匀浆和血液中检出卵黄蛋白原,50、100和300μg/L组卵黄蛋白原含量随辛基酚浓度的增大而显著增大,500μg/L组卵黄蛋白原含量低于300μg/L组、高于100μg/L组,均为极显著差异(P<0.01)。
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| 关 键 词： | 鲤 辛基酚 雌激素效应 卵黄蛋白原 |
| 收稿时间： | 2009/7/19 0:00:00 |
| 修稿时间： | 2009/11/27 0:00:00 |
Estrogenic effects of octylphenol on carp (Cyprinus carpio) |
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| GONG Xianghon,XU Yingjiang,RENG Lihu,ZHANG Shijuan,ZHANG Xiuzhen and ZHANG Limin.Estrogenic effects of octylphenol on carp (Cyprinus carpio)[J].Journal of Fisheries of China,2010,34(3):410-414. |
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| Authors: | GONG Xianghon XU Yingjiang RENG Lihu ZHANG Shijuan ZHANG Xiuzhen and ZHANG Limin |
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| Institution: | GONG Xiang-hong,XU Ying-jiang,REN Li-hua,ZHANG Shi-juan,ZHANG Xiu-zhen,ZHANG Li-min (Marine Fisheries Research Institute of Sh,ong Province,Yantai 264006,China ) |
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| Abstract: | The aim of the study was to investigate the estrogenic effects of octylphenol (OP) on carp (Cyprinus carpio). Vitellogenin (Vtg) and the parameters of growth and development were determined as biomarkers. The one-year-old carp from a large reservoir were kept in the laboratory for 7 days. Then they were exposed to OP under semi-static conditions for 32 days. They were fed once a day. The water temperature was (18±1) ℃ during the experiment. Five concentrations of OP at 10, 50,100,300 and 500 μg/L were selected for this study. The mortality, abnormal behavior and appearance of the carp were recorded during the exposure. At the end of the exposure, fish were sampled to measure body weight, body length and gonadal weight, then plasma and liver homogenates were prepared for the determination of Vtg concentration by ELISA. The data were analysed with SPSS 11.5. At the end of exposure, it was found that there was no significant difference in the survival and growth of the fish. The exposure to OP caused obvious changes in gonadosomatic index (GSI). In accordance with the increase of the OP concentration,the GSIs of female carp increased, while that of male carp decreased. OP at 10 μg/L caused the GSIs of male carp decreased significantly (P<0.05). The GSIs of female carp increased significantly when the OP concentration reached 50 μg/L (P<0.05). OP induced the synthesis of Vtg in male carp. Vtg was not detected in plasma or liver homogenates in control groups, and it was detected in some of the samples in 10 μg/L group. The Vtg levels increased greatly when the OP concentration reached 50 μg/L (P<0.05).The Vtg levels in 500 μg/L group were significantly lower than those in 300 μg/L (P < 0. 01), but significantly higher than those in 100 μg/L group (P <0.01). The results indicated that octylphenol (OP) has obvious impact on the gonadal development of carps. It can induce Vtg synthesis in male carps. OP has visible estrogenic effects on carp (Cyprinus carpio) although it did not affect the growth of the fish at low exposure concentrations. |
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| Keywords: | Cyprinus carpio octylphenol estrogenic effect vitellogenin |
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