| 棕点石斑鱼Piscidin样肽基因的克隆及其成熟肽的原核表达 |
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| 作者姓名： | 陈大玮 邓利 何凡 邱聪龄 马一见 刘志刚 |
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| 作者单位： | 1. 深圳大学生命科学学院,518060
2. 深圳大学医学院,518060 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目(2006AA100308); 广东省大学生创新实验项目(1059010021)共同资助 |
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| 摘 要： | 以简并引物通过RT-PCR从重要经济海水鱼类棕点石斑鱼Epinephelus fuscoguttatus白细胞中扩增出一条388bp具有完整编码框的cDNA序列。Blast分析初步显示,该序列属于鱼类抗菌肽Piscidin家族的一员,将其命名为棕点石斑鱼Piscidin样肽(GU592793),推导氨基酸序列具有界定鱼类Piscidin的以下共同特征:(1)预测的信号肽序列与其他Piscidin的同源性较高,在60%~95%之间;(2)推测的成熟肽N端6个氨基酸残基富含Phe、Ile及His;(3)成熟肽第8个和第13个氨基酸位点均为Gly。NJ进化树分析表明,该cDNA推导的氨基酸序列与赤点石斑鱼Epinephelusakaarapiscidin-like peptide(ACE78290)、斜带石斑鱼Epinephelus coioidesPiscidin-like peptide(ACE78291)以高达81%的支持率聚为一支。也表明本研究成功克隆到棕点石斑鱼首条PiscidincDNA序列。推测的成熟肽为两亲性阳离子肽,等电点12.48,Schiffer-Edmunson预测其二级结构呈α螺旋构型。将...
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| 关 键 词： | 抗菌肽 Piscidin 基因克隆 原核表达 棕点石斑鱼 |
| 收稿时间： | 2010/10/17 0:00:00 |
| 修稿时间： | 2010/12/22 0:00:00 |
Cloning of antimicrobial piscidin-like peptide and prokaryotic expression of the putative mature peptide in Epinephelus fuscoguttatus |
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| Authors: | CHEN Da-wei DENG Li HE Fan QIU Cong-ling MA Yi-jian LIU Zhi-gang |
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| Institution: | CHEN Da-wei1 DENG Li1 HE Fan1 QIU Cong-ling1MA Yi-jian1 LIU Zhi-gang2(1College of Life Sciences,Shenzhen University,518060)(2Shenzhen University,School of Medicine,518060) |
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| Abstract: | A 388bp cDNA sequence with complete open reading frame was amplified by RT-PCR with degenerate primers from leucocytes of Epinephelus fuscoguttatus,an important commercial marine fish species.Blast analysis preliminarily suggested that the obtained sequence,which was named as Epinephelus fuscoguttatus piscidin-like peptide(GU592793),is a member of fish antimicrobial peptide piscidin family.The deduced amino acid sequence was found to have the common features for defining the fish piscidin,including:(1) sign... |
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| Keywords: | Antimicrobial peptide Piscidin Molecular cloningProkaryotic expression Epinephelus fuscoguttatus |
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